Modern Concepts and Methods in Bioenergetics

Modern Concepts and Methods in Bioenergetics examines mitochondrial and cellular bioenergetics, including advances made in understanding the contribution of dysfunctional bioenergetics in the pathogenesis and propagation of various diseases, how to target pathways with novel drugs and metabolites to prevent or treat diseases, and the use of mitochondrial bioenergetic signatures as biomarkers for disease progression. This book highlights methods that have been used to gauge the Mitochondrial Health Index, and also provides novel approaches utilized to identify how mitochondria participate in cellular signaling, serve as a platform for information transmission and act as a sink for intra- and extracellular reactive oxygen species.

These topics are discussed along with central themes of mitochondrial bioenergetics and redox biology, factors that are integral for optimal cellular health.

• Provides practical methods and protocols instruction, empowering new research and clinical translation
• Features chapter contributions from leading, international researchers in the field
• Emphasizes new and evolving studies in the field of bioenergetics

1. Introduction

Part 1: Mitochondrial Bioenergetics and the Cellular Redoxome 2. Mitochondrial bioenergetics and the respiratory chain 3. Supercomplex assemblies 4. Mitochondria as dynamic sources and sinks for hydrogen peroxide 5. Nicotinamide nucleotide transhydrogenase in health and disease 6. Mitochondria: Essential redox signalling platforms 7. Sex differences in mitochondrial bioenergetics and redox buffering capacities

Part 2: Mitochondria in Health and Disease 8. Targeting brown fat mitochondria for the treatment of metabolic diseases 9. The Bioenergetics Health Index as a biomarker for disease progression 10. The gut microbiome-mitochondria signalling axis 11. Mitochondria as central hubs for one-carbon metabolism 12. Mitochondrial dysfunction in the development of fatty liver disease 13. Mitochondrial DNA mutations in human diseases 14. The importance of reverse electron transport in the respiratory chain in physiology and disease progression 15. Mitochondrial dynamics and mitochondrial vesicle formation 16. Mitochondria targeted pharmaceuticals for the treatment of diseases

Part 3: Methods in the Study of Mitochondrial Bioenergetics 17. Interrogation of mitochondrial bioenergetics in permeabilized muscle fibers 18. Methods for examining the bioenergetics of mitochondria in cultured cells 19. Measurement of bioenergetics using isolated mitochondria; from the Clark-type electrode to more modern instruments 20. Methods for interrogating mitochondrial ROS production 21. An experimental tool kit for quantifying the native rates of ROS production by the twelve individual sites of production 22. Approaches for examining mitochondrial supercomplex assemblies

Manisha Singh, PhD, is a trained human and mouse immunologist and during the last 10 years, she has specialized in cancer immunology. The main focus of her work has been to increase the efficacy and understand the molecular mechanisms of various cancer immunotherapies. She is serving as the principal investigator on a 2-year grant ($545,486), having been funded by the US Department of Defense. Dr. Singh's current research is focused on understanding the role of inflammation in immune suppression and resistance to immunotherapy in cancer. Recently, she has submitted the NIH-R01 grant in which she proposed to study the role of IL-1a in immunotherapy induced resistance. She has published 24 peer-reviewed articles and many abstracts in prestigious journals such as Nature Communication, Journal of Immunology, and Journal of Clinical Investigator. Her work has been cited by several prestigious, peer-reviewed, international journals.