Mitosis Methods and Protocols

This detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, including chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis.- Methods for Investigating Cell Division Mechanisms in C. elegans.- Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes.- Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies.- Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells.- Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells.- Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin.- Using Microinjection of Mammalian Cultured Cells to Study Cell Division.- In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments during Mitosis.- Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity.- Chemical Biology of Mitotic Spindle Assembly Motors.- Deploying Kinase Inhibitors to Study Pediatric Gliomas.- Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells.- Degron Tagging Using mAID and SMASh Tags in RPE-1 Cells.- Centromere Tension Measurement in Budding Yeast Mitosis.- Simulating Dynamic Chromosome Compaction: Methods for Bridging In Silico to In Vivo.- Using FLIM-FRET for Characterizing Spatial Interactions in the Spindle.- Imaging Mitosis with Lattice LightSheet.