Mitochondrial Medicine Volume 3: Manipulating Mitochondria and Disease- Specific Approaches

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease.- Mitochondrial Transplantation for Ischemia Reperfusion Injury.- Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device.- Lipophilic Conjugates for Carrier-Free Delivery of RNA Importable Into Human Mitochondria.- Drug Discovery Assay to Identify Modulators of the Mitochondrial Ca²⁺ Uniporter.- Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice.- Electron Attachment to Isolated Molecules as a Probe To Understand Mitochondrial Reductive Processes.- Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation.- Assessment of the Effects of Drugs on Mitochondrial Respiration.- The Ndufs4 Knockout Mouse: A Dual Threat Model of Childhood Mitochondrial Disease and Normative Aging.- Suborganellar Localisation of Mitochondrial Proteins and Transcripts in Human Cells.- In vivo Assessment of Mitochondrial Oxygen Consumption.- Update of Mitochondrial Network Analysis by Imaging: Proof of Technique in Schizophrenia.- Heterologous Inferential Analysis (HIA) and other Emerging Concepts In Understanding Mitochondrial Variation in Pathogenesis: There is no More Low Hanging Fruit.- Accurate Measurement of Cellular and Cell-Free Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR.- Measurement of Mitochondrial Respiration in Platelets.- Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria.- Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells.- Single Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease.- A New Method for Sequencing the Mitochondrial Genome by Using Long Read Technology.- Mitochondrial DNA as a Sensitive Biomarker of UV-induced Cellular Damage in Human Skin.- Isolation of Mitochondria-associated ER Membranes (MAMs), Synaptic MAMs and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells.- Mitochondrial Diagnostics: A Discovery-Based Biochemical Platform for Phenotyping Human Peripheral Blood Cell Mitochondria.- Bioenergetic Profiling Of Human Pluripotent Stem Cells.- Integrative Methods for Studying Cardiac Energetics.- Isolation of Mitochondria from Retinal Pigment Epithelial Cell Cultures and an Application Of High Resolution Respirometric Assay (XF^e96 Seahorse assay).- The Isolation and Deep Sequencing of Mitochondrial DNA.- Ultrastructure of the Mitochondria-Associated Membranes in Human Tumor Specimens.