MicroRNA and Cancer Methods and Protocols

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field

Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics.- MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential.- Involvement of miRNAs and Pseudogenes in Cancer.- microRNAs Reprogram Tumor Immune Response.- APOBEC Gene Expression Regulated by microRNAs.- MicroRNAs Changes the Landscape of Cancer Resistance.- MicroRNA, Noise, and Gene Expression Regulation.- Deep Sequencing Reveals a microRNA Expression Signature in Triple-negative Breast Cancer.- Detection of Plasma microRNA Signature in Osteosarcoma Patients.- Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells.- Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy.- Evaluation of microRNA Delivery in vivo.- Angiogenesis Analysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian Cancer.- Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data.- Construction of Multi-potent microRNA Sponge and its Functional Evaluation.- microRNA Sequencing Data Analysis Toolkits.