Methods in Reproductive Aquaculture Marine and Freshwater Species

The large amount of information on fish reproduction available is not always readily accessible to all interested parties. Written to appeal to aquaculturalists, conservation managers, and scientific researchers, Methods in Reproductive Aquaculture provides an overview of available techniques and addresses ways to improve depleted stocks of endangered fish and other species. The book focuses on gamete quality and management, exploring new objectives and areas of application and research, and new tools to deal with some aspects in reproduction of aquatic species. It begins with an examination of basic methods and techniques for gamete extraction, mainly sperm collection and egg stripping in different species, then describes techniques of spawning stimulation in males and females. The volume then highlights sperm and egg quality evaluation and considers sperm analysis from a practical broodstock management point of view and in the selection of appropriate breeders. The book goes on to discuss techniques used for artificial fertilization and the procedures for obtaining modified offspring. It introduces cryopreservation procedures for sperm, oocytes, and embryos, then discusses the materials and facilities required for gamete preparation and freezing-thawing procedures. The book concludes with an extensive section detailing sperm cryopreservation protocols for fifty-six marine and freshwater species. Unique in its focus on both marine and freshwater aquaculture of fishes, the book provides information on endangered, highly profitable species for aquaculture or fisheries and species with high potential in laboratory research. Presenting the available information in an easily understandable way, this is not merely a techniques book, but also a complete guide to fish reproduction, gamete quality, and management.

GAMETE EXTRACTION TECHNIQUES Reproduction and control of ovulation, spermiation and spawning in cultured fish, E. Mañanós, N. Duncan, and C. Mylonas Methods for sperm collection, J-C. Gwo SPERM AND EGG QUALITY EVALUATION Sperm quality assessment, E. Cabrita, V. Robles, and P. Herráez Egg quality determination in teleost fish, F. Lahnsteiner, F. Soares, L. Ribeiro, and M.T. Dinis ARTIFICIAL FERTILIZATION IN AQUACULTURED SPECIES:FROM NORMAL PRACTICE TO CROMOSOME MANIPULATION Artificial fertilization in aquaculture species: from normal practice to chromosome manipulation, B. Urbányi , Á. Horváth, and Z. Bokor SPERM AND EGG CRYOPRESERVATIONChilled storage of sperm and eggs, J. Bobe and C. Labbé Basic principles of fish spermatozoa cryopreservation, J. Cloud and S. Patton Cryopreservation of fish oocytes, T. Zhang and E. Lubzens Embryo cryopreservation: what we know until now, V. Robles, E. Cabrita, J.P. Acker, and P. Herráez Cryobiological material and handling procedures, F. Martínez-Pastor and S.L. Adams PROTOCOLS FOR SPERM CRYOPRESERVATION Cryopreservation of sperm of loach (Misgurnus fossilis), J. Kopeika, E. Kopeika, T. Zhang, and D. Rawson Zebrafish Sperm Cryopreservation with N, N-Dimethylacetamide, J.P. Morris IV, A. Hagen, and J.P. Kanki Sperm cryopreservation of live-bearing fishes of the Genus Xiphophorus, Q. Dong, C. Huang, L. Hazlewood, R.B. Walter, and T.R. Tiersch Use of post-thaw silver carp (Hypophtalmichthys molitrix) spermatozoa to increase hatchery productions, B. Alvarez, A. Arenal, R. Fuentes, R. Pimentel, Z. Abad, and E. Pimentel Cryopreservation of common carp sperm, B. Urbányi and Á. Horváth Sperm cryopreservation from sea perch, Lateolabrax japonicas, S-L. Chen, X.S. Ji, and Y-S. Tian Semen cryopreservation of piracanjuba (Brycon orbignyanus), an endangered Brazilian species, A.T.M. Viveiros and A.N. Maria Cryopreservation of endangered Formosan landlocked salmon (Oncorhynchus masou formosanus) semen, J-C. Gwo, H. Ohta, K. Okuzawa, L-Y. Liao, and Y-F. Lin Protocols for the cryopreservation of Salmonidae semen, Lota lota (Gadidae) and Esox lucius (Esocidae), F. Lahnsteiner and N. Mansour Rainbow trout (Oncorhynchus mykiss) sperm cryopreservation, P. Herráez, V. Robles, and E. Cabrita Sperm cryopreservation of sex-reversed rainbow trout (Oncorhynchus mykiss), V. Robles, E. Cabrita, and P. Herráez Cryopreservation of testicular sperm from European catfish (Silurus glanis), G. Maisse, B. Ogier de Baulny, and C. Labbé Semen Cryopreservation of the African catfish, Clarias gariepinus, A.T.M. Viveiros and J. Komen Cryopreservation of sperm from species of the order Acipenseriformes, Á. Horváth, B. Urbányi, and S.D. Mims Different protocols for the cryopreservation of European eel (Anguilla anguilla) sperm, J.F. Asturiano Cryopreservation of Striped Bass Morone saxatilis Spermatozoa, L.C.Woods III, S. He, and K. Jenkins-Keeran Cryopreservation of semen from striped trumpeter (Latris lineata), A.J. Ritar Cryopreservation of Atlantic halibut sperm, Hippoglossus hippoglossus, I. Babiak Sperm cryopreservation: an actual tool for Seabass (Dicentrarchus labrax) reproduction and breeding control in research and production, C. Fauvel and M. Suquet Notes on Diplodus puntazzo sperm cryopreservation, F. Barbato, S. Canese, F. Moretti, A. Taddei, A. Fausto, L. Abelli, M. Mazzini, and K.J. Rana Sperm Cryopreservation from the Marine Teleost, Sparus aurata, E. Cabrita, V. Robles, C. Sarasquete, and P. Herráez Cryopreservation of seabream semen, J-C. Gwo Cryopreservation of sperm from winter flounder, Pseudopleuronectes americanus, R.M. Rideout, I.A.E. Butts, and M.K. Litvak Cryopreservation of sperm in turbot (Psetta maxima), M. Suquet, O. Chereguini, and C. Fauvel Cryopreservation of grouper semen, J-C. Gwo and H. Ohta Cryopreservation of sperm from Atlantic cod (Gadus morhua) andhaddock (Melanogrammus aeglefinus), R.M. Rideout and D.L. Berlinsky Cryopreservation of small abalone (Haliotis diversicolor supertexa) semen, J-C. Gwo Cryopreservation of Pacific Oyster Sperm, Q. Dong, C. Huang, B. Eudeline, and T. R. Tiersch A simple method for freezing Pacific oyster (Crassostrea gigas) sperm in quantities suitable for commercial hatcheries, S.L. Adams, J.F. Smith, R.D. Roberts, A.R. Janke, H.F. Kaspar, H.R. Tervit, P.A. Pugh, S.C. Webb, and N.G. King Cryopreservation of Eastern Oyster Sperm, C.G. Paniagua-Chavez and T.R. Tiersch Cryopreservation of sea urchin (Evechinus chloroticus) sperm, S.L. Adams, P.A. Hessian, and P.V. Mladenov Protocol for cryopreservation of Penaeus vannamei sperm cells, M. Salazar, M.Lezcano, and C.Granja Techniques for cryopreservation of spermatophores of the giant freshwater prawn, Macrobrachium rosenbergii (de Man), P. Damrongphol and K. Akarasanon Protocol development for the cryopreservation of spermatozoa and spermatophores of the mud crab, Scylla serrata, S. Bhavanishankar and T. Subramoniam Index