Methods in Cell Wall Cytochemistry

Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.)Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.

PrefaceCell Wall-Morphology & Chemical CompositionCarbohydratesCellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated PolysaccharidesGlycoproteinsStructural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like ProteinsEnzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and RibonucleusesLipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and TryphineLignin and Other Phenolic Substances-Lignins, Phenolic Acids and AmidesMineral Substances-Silica, Calcium, and BoronLight Microscopic CytochemistryIntroductionTissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of MaterialsStains and Staining-Introduction, Control Procedures, Incubation of MaterialsMounting of Stained MaterialsProcedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing ProceduresProcedures for the Cytochemical Localizations of Different Cell Wall SubstancesCarbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharidesProteins-Non-Specific Cell Wall Proteins, Enzymic ProteinsLigninsSporopolleninsCutins & SuberinMineralsFluorescent Microscopic CytochemistryIntroductionFluorescent Cytochemical MethodsCell Wall CarbohydratesCell Wall ProteinsLigninFerulic AcidSporopolleninCutin and SuberinTransmission Electron Microscopic CytochemistryIntroductionSpecimen PreparationElectron Microscopic StainingTEM Cytochemical MethodsCell Wall CarbohydratesCell Wall ProteinsCutin and SuberinLectin CytochemistryIntroductionProcedures Involving Lectin ProbesImmunocytochemistryIntroductionBasics of Immunocytochemistry-Immunization, Storing and Purifying Antibodies, Labeling of Antibodies, Tissue Preparation, Immunolabeling, Mounting of SectionsMethods in Immunocytochemistry-Methods in Antibody Production and Tissue Preparation, Application of Immunocytochemistry to Study Cell Wall ComponentsLiterature Cited (at the end of each Chapter)

Krishnamurthy, K V