Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols

MRSA Protocols for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose and then goes into detailed protocols for every step of analysis. Several chapters also include a section with tips on individual steps not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting.

Among the topics discussed in this volume are Staphylococcus aureus infections, MIC analysis, ITS-PCR identification, SCCmec analysis, and Biofilm formation. The book also contains helpful case studies, and authors provide insights into the control, prevention, and treatment of infections.

Oriented towards routine clinical diagnosis, research, and actual practice for treatment of patients infected by MRSA, MRSA Protocols for Methods in Molecular Biology will make a useful basis for anyone interested in instituting a new method for studying MRSA.

CONTENTS Chapter 1 Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections / Elizabeth Palavecino Chapter 2 MRSA Case Studies / Kurt D. Reed, Mary E. Stemper, and Sanjay K. Shukla Chapter 3 MIC analysis and susceptibility testing of MRSA / German Bou Chapter 4 ITS-PCR identification of MRSA / Shin-ichi Fujita Chapter 5 Pulsed-field Gel Electrophoresis of Methicillin-resistant Staphylococcus aureus / Kurt D. Reed, Mary E. Stemper, and Sanjay K. Shukla Chapter 6 Multilocus sequence typing (MLST) of Staphylococcus aureus / Nicholas A Saunders and Anne Holmes Chapter 7 Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA / Teruyo Ito, Kyoko Kuwahara, and Keiichi Hiramatsu Chapter 8 Targeted gene disruption for the analysis of virulence of Staphylococcus aureus / J. Ross Fitzgerald Chapter 9 Molecular Analysis of Staphylococcal Superantigens / Patrick M. Schlievert and Laura C. Case Chapter 10 Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus / James E. Cassat, Chia Y. Lee, and Mark S. Smeltzer Chapter 11 Comparative analysis of staphylococcal adhesion and internalization by epithelial cells / Xudong Liang and Yinduo Ji Chapter 12 Comparative analysis of MRSA / Fumihiko Takeuchi, Tadashi Baba, and Keiichi Hiramatsu Chapter 13 Genomic analysis of gene expression of Staphylococcus aureus / Chuanxin Yu, Junsong Sun, Li Zheng, and Yinduo Ji Chapter 14 Proteomic approach to investigate Methicillin-Resistant Staphylococcus aureus (MRSA) / Patrice Francois, Alexander Scherl, Denis Hochstrasser, Jacques Schrenzel Chapter 15 Environmental surveillance for methicillin-resistant Staphylococcus aureus / J Scott Weese Chapter 16 The Control and Prevention of MRSA Infections / Liangsu Wang and John F. Barrett Chapter 17 Treatment of Infections Due to Resistant Staphylococcus aureus / Gregory M. Anstead, Gabriel Quinones-Nazario, and James S. Lewis II