Metabonomics Methods and Protocols

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS). Chapters focus on technologies and chemometrics, generation of metabonomics data, extraction of meaningful information from data, drug development, toxicology, diagnostics, and describing metabonomics as an essential part of systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Metabonomics - Analytical Techniques and Associated Chemometrics at a Glance.- Sample Collection and Preparation of Biofluids and Extracts for NMR Spectroscopy.- NMR Spectroscopy of Biofluids and Extracts.- High-resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue.- Sample Collection and Preparation of Biofluids and Extracts For Liquid Chromatography-Mass Spectrometry.- Liquid Chromatography-Mass Spectrometry of Biofluids and Extracts.- Sample Collection and Preparation of Biofluids and Extracts For Gas-Chromatography-Mass Spectrometry.- Gas Chromatography-Mass Spectrometry of Biofluids and Extracts.- Capillary Electrophoresis Mass Spectrometry.- Preprocessing of Raw Metabonomics Data.- Extracting Meaningful Information From Metabonomics Data Using Multivariate Statistics.- Combining Metabonomics and Other -omics Data.- The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry With Particular Focus on Metabolomics Research.- Metabonomics and Drug Development.- Metabonomics and Toxicology.- Metabonomics and Diagnostics.- Metabonomics and Systems Biology.