Metabolomics Methods and Protocols

Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies.

Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry.- Metabolite Profiling in Blood Plasma.- Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles.- Metabolomic Profiling of Natural Volatiles.- Metabolomics: Data Integration and Data Mining.- Integrative Profiling of Metabolites and Proteins.- Integrating Profiling Data.- Visualization and Analysis of Molecular Data.- A Gentle Guide to the Analysis of Metabolomic Data.- Capillary Electrophoresis Coupled to Mass Spectrometry.- Capillary Electrophoresis-Mass Spectrometry for Metabolomics.- Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation.- Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics.- Electrochemical Detection of Metabolites.- High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors.- Metabolic Fluxes.- Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data.- Understanding the Roadmap of Metabolism by Pathway Analysis.- NMR Metabolome Analysis.- Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy.- Nuclear Magnetic Resonance Metabonomics.- Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics.