Maternal Placental Interface Methods and Protocols

Authoritative and cutting-edge, Maternal Placental Interface: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Histological analysis of trophoblast cells in the mouse placental labyrinth zone.- Isolation and Immunophenotyping of Leukocytes from the Human Maternal-Fetal Interface.- Evaluation of leukocyte chemotaxis induced by human fetal membranes in an in vitro model.- In vitro culturing of Human Term Placental Explants.- In vitro culturing of Human Trophoblasts from Term Placenta.- Culture of human fetal membranes in a two independent-compartment model: an ex vivo approach.- Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae.- Generation of knockout mouse trophoblast stem cells by CRISPR/Cas.- CRISPR-activation in mouse trophoblast stem cells.- Feto-maternal interface organ-on-chip: a new technology to study ascending infection.- Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-Maternal Interface.- Placental trophoblast cell isolation from the term placenta.- Dissociation of placental tissues for single-cell techniques.- Immunophenotyping of leukocytes in amniotic fluid.- Obtainig of tissue of human amniotic membrane and identification of pluripotency markers by immunofluorescence.- DNA isolation from fresh placenta, in formalin and paraffin.- Infection of fetal membranes with Mycobacterium tuberculosis and tissue processing to isolate RNA for expression analysis.