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bhattacharya sanjoy k. (curatore) - lipidomics

Lipidomics Methods and Protocols

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Lingua: Inglese


Pubblicazione: 01/2023
Edizione: 2nd ed. 2023


This fully updated book presents an account of areas of utility, techniques, and bioinformatic advancements in the field of lipidomics. Beginning with protocols for lipid isolation and extraction, the volume continues with techniques from extractive mass spectrometry to imaging mass spectrometry methods allowing localization of lipids in tissues. These protocols have been complemented by methods addressing specific problems from membranes, fractionated subcellular compartments or organelles to whole organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Lipidomics: Methods and Protocols, Second Edition serves as an ideal guide for biochemists, molecular biologists, neuroscientists, vision research scientists, as well as all biomedical researchers with interest in disease discovery and drug development.


1. Isolation of Mitochondrial Lipids and Mass Spectrometric Analysis

            Alexa M. Jauregui, Zoé M. Cubero Cortés, Sean D. Meehan, and Sanjoy K. Bhattacharya


2. Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation

            Blake R. Hopiavuori, Dustin R. Masser, Joseph L. Wilkerson, Richard S. Brush, Nawajes A. Mandal, Robert E. Anderson, and Willard M. Freeman


3. Multi-Macromolecular Extraction from Endosymbiotic Anthozoans

            Anderson B. Mayfield


4. High Resolution Liquid Chromatography-Mass Spectrometry for Lipidomics

            Faith Christine Harvey, Vanessa Collao, and Sanjoy K. Bhattacharya


5. Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry

            Jennifer Arcuri and Sanjoy K. Bhattacharya


6. Protocol and Methods Applicable to Retinal Vascular Diseases

            Saurabh Kumar, Manjunath B. Joshi, and Inderjeet Kaur


7. Analysis and Annotation of Phospholipids by Mass Spectrometry-Based Metabolomics

            Álvaro González-Domínguez, María Santos-Martín, Ana Sayago, Alfonso María Lechuga-Sancho, Ángeles Fernández-Recamales, and Raúl González-Domínguez


8. Profiling the Mammalian Lipidome by Quantitative Shotgun Lipidomics

            Mads Møller Foged, Kenji Maeda, and Mesut Bilgin


9. Lipidomics Analysis with Triple Quadrupole Mass Spectrometry

            Meher A. Saleem, Anna Mueller, and Sanjoy K. Bhattacharya


10. High-Performance Chromatographic Separation of Cerebrosides

            Renaud Sicard and Ralf Landgraf


11. Quantification of Endocannabinoids from Biological Samples Using Liquid Chromatography Tandem Mass Spectrometry

            Zhiying Wang, Chenglin Mo, Lynda Bonewald, and Marco Brotto


12. Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains

            Martin-Paul Agbaga, Mark E. McClellan, and Michael H. Elliott


13. Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry

            Emily J. Neag, Vanessa Collao, and Sanjoy K. Bhattacharya


14. Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Using Imaging Mass Spectrometry

            Varun Krishnan, Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya


15. A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipid Droplets

            Margaret Gurumani, Shamroop Mallela, Javier Varona, Sandra Merscher, Alessia Fornoni, and Hassan Al-Ali


16. Capillary Electrophoresis Plasma Fractionation for Lipids Analysis

            Anddre Osmar Valdivia, Carolyn Cray, and Sanjoy K. Bhattacharya


17. Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for the Analysis of (Phospho)Lipids

            Jenny Leopold, Kathrin M. Engel, Patricia Prabutzki, and Jürgen Schiller


18. Single Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa

            Pallavi Lahiri, Priyakshi Gogoi, and Dipankar Ghosh


19. Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells

            Evan B. Stubbs, Jr.


20. Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate

            Joseph L. Wilkerson, Sandip K. Basu, and Nawajes Mandal


21. Lipid Extraction and Sample Preservation Techniques for Stable Isotope Analysis and Ecological Assays

            Kyle H. Elliott, James D. Roth, Kevin Crook, and David Yurkowski


22. Rapid Analysis of Plasmalogen Individual Species by High-Resolution Mass Spectrometry

            Glenda Vasku, Niyazi Acar, and Olivier Berdeaux


23. Untargeted Analysis of Lipids Containing Very Long Chain Fatty Acids in Retina and Retinal Tight Junctions

            Julia V. Busik, Hannah Decot, Anna B. Lin, and Todd A. Lydic


24. Analysis of Lipid Contents in Human Trabecular Meshwork Cells by Multiple Reaction Monitoring (MRM) Profiling Lipidomics

            Ting Wang and Padmanabhan Paranji Pattabiraman


25. Mass Spectrometry Approaches for Detection and Determination of Prostaglandins from Biological Samples

            Zhiying Wang, Chenglin Mo, Kamal Awad, Lynda Bonewald, and Marco Brotto


26. Downloading and Analysis of Metabolomic and Lipidomic Data from Metabolomics Workbench Using MetaboAnalyst 5.0

            Ashley Howell and Charles Yaros


27. Accurate Analysis of Lipid Building Blocks Using the Tool LipidOne

            Roberto Maria Pellegrino, Matteo Giulietti, Husam B.R. Alabed, Anna Aurora Taddei, Sandra Buratta, Lorena Urbanelli, Francesco Piva, and Carla Emiliani


28. Image Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate

            Sandip K. Basu and Nawajes Mandal


29. Characterization of Trabecular Meshwork Mechanical Property Modulation after Application of Lipids

            Wyndham Bachelor, Jennifer Arcuri, Sanjoy K. Bhattacharya, and Noël M. Ziebarth


30. C-Laurdan: Membrane Order Visualization of HEK293t Cells by Confocal Microscopy

            Sean D. Meehan, Colin Hayter, and Sanjoy K. Bhattacharya


31. Sonication-Based Basic Protocol for Liposome Synthesis

            Roberto Mendez

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 932 gr
Formato: Copertina rigida
Illustration Notes:XVI, 374 p. 93 illus., 52 illus. in color.
Pagine Arabe: 374
Pagine Romane: xvi

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