Ion Mobility-Mass Spectrometry - Paglia Giuseppe (Curatore); Astarita Giuseppe (Curatore) | Libro Humana 11/2020 -

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paglia giuseppe (curatore); astarita giuseppe (curatore) - ion mobility-mass spectrometry

Ion Mobility-Mass Spectrometry Methods and Protocols


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Lingua: Inglese


Pubblicazione: 11/2020
Edizione: 1st ed. 2020


1. Fundamentals of Ion Mobility-Mass Spectrometry for the Analysis of Biomolecules

            Caleb B. Morris, James C. Poland, Jody C. May, and John A. McLean


Part I: Metabolomics and Lipidomics


2. Utilizing Drift Tube Ion Mobility Spectrometry for the Evaluation of Metabolites and Xenobiotics

            Melanie T. Odenkirk and Erin S. Baker


3. Untargeted Differential Metabolomics Analysis Using Drift Tube Ion Mobility-Mass Spectrometry

            Rick Reisdorph, Cole Michel, Kevin Quinn, Katrina Doenges, and Nichole Reisdorph


4. Drift Tube Ion Mobility-Mass Spectrometry for Non-Targeted 'Omics

            Tim J. Causon, Ruwan T. Kurulugama, and Stephan Hann


5. The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines

            Nicholas B. Vera, Michelle Clasquin, Stephen L. Coy, and Paul Vouros


6. Traveling Wave Ion Mobility-Mass Spectrometry to Enhance the Detection of Low Abundance Features in Untargeted Lipidomics

            Zeeshan Hamid and Andrea Armirotti


7. Lipidomics by HILIC-Ion Mobility-Mass Spectrometry

            Amy Li, Kelly M. Hines, and Libin Xu


8. A Workflow for the Identification of Mycotoxin Metabolites Using Liquid Chromatography–Ion Mobility-Mass Spectrometry

            Laura Righetti and Chiara Dall’Asta


9. Quantitation of Cyclosporin A in Cell Culture Media by Differential Mobility Mass Spectrometry (DMS-MS/MS)

            Amol Kafle, James Glick, Stephen L. Coy, and Paul Vouros


Part II: Proteomics and Glycomics


10. An Analytical Perspective on Protein Analysis and Discovery Proteomics by Ion Mobility-Mass Spectrometry

            Johannes P.C. Vissers and Michael McCullagh


11. Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics

            Lauren J. Tomlinson and Claire E. Eyers


12. Liquid Extraction Surface Analysis (LESA) High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) Mass Spectrometry for In Situ Analysis of Intact Proteins

            Rian L. Griffiths, Klaudia I. Kocurek, and Helen J. Cooper


13. Ion Mobility-Mass Spectrometry of Glycoconjugates

            Weston B. Struwe and David J. Harvey


Part III: Ambient Ionization and Imaging Ion Mobility Mass Spectrometry


14. Desorption Atmospheric Pressure Photoionization Coupled with Ion Mobility-Mass Spectrometry

            Tiina J. Kauppila


15. Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation

            Sylwia A. Stopka and Akos Vertes


16. Matrix-Assisted Laser Desorption and Desorption Electrospray Ionization Mass Spectrometry Coupled to Ion Mobility

            James I. Langridge and Emmanuelle Claude


Part IV: Bioinformatic Solutions for Analyzing IM-MS Data and Deriving CCS Values


17. The Use of LipidIMMS Analyzer for Lipid Identification in Ion Mobility-Mass Spectrometry-Based Untargeted Lipidomics

            Xi Chen, Zhiwei Zhou, and Zheng-Jiang Zhu


18. MolFind2: A Protocol for Acquiring and Integrating MS3 Data to Improve In-Silico Chemical Structure Elucidation for Metabolomics

            Milinda A. Samaraweera, Dennis W. Hill, and David F. Grant


19. Collision Cross Section Calculations Using HPCCS

            Gabriel Heerdt, Leandro Zanotto, Paulo C.T. Souza, Guido Araujo, and Munir S. Skaf


This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ion Mobility-Mass Spectrometry: Methods and Protocols serves as an ideal resource for scientists delving into the technique's unprecedented analytical advantages, enabling novel qualitative and quantitative applications for the analysis of complex biological samples.

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 618 gr
Formato: Brossura
Illustration Notes:19 Illustrations, black and white
Pagine Arabe: 312
Pagine Romane: xi

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