Invariant Natural Killer T-Cells Methods and Protocols

This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Genetic Analysis of iNKT Cell Development and Function.- Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells.- Retroviral Transduction of NKT Hybridoma Cells.- Methods for Studying Mouse and Human Invariant Natural Killer T-Cells.- Isolation and Detection of Murine iNKT Cells in Different Organs.- Identifying, Isolation, and Functional Use of Human Liver iNKT Cells.- Isolation and Characterization Methods of Human Invariant NKT Cells.- Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry.- Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry.- iNKT Cell Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach.- Expansion of Human iNKT Cells Ex Vivo.- The Expansion and Cytotoxicity Detection of Human iNKT Cells.- Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood.- Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver UsingCXCR6-eGFP Transgenic Mice.- In Vivo Cytotoxicity by a-GalCer-Transactivated NK Cells.- Redirecting iNKT Cell Anti-Tumor Immunity with aGalCer/CD1d-scFv Fusion Proteins.- Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles during Development.