Introduction to Optical Microscopy

This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.

1. Introduction; 2. Monochromatic wave propagation; 3. Monochromatic field propagation through lens; 4. Intensity propagation; 5. 3D imaging; 6. Radiometry; 7. Intensity fluctuations; 8. Detection noise; 9. Absorption and scattering; 10. Widefield microscopy; 11. Interference microscopy; 12. Optical coherence tomography; 13. Fluorescence; 14. Confocal microscopy; 15. Structured illumination microscopy; 16. Multiphoton microscopy; 17. Multiharmonic microscopy; 18. Pump-probe microscopy; 19. Superresolution; 20. Imaging in scattering media; Appendix A. Properties of Fourier transforms; Appendix B. Miscellaneous math; Appendix C. Jones matrix description of polarization.

Jerome Mertz is a Professor of Biomedical Engineering and Director of the Biomicroscopy Laboratory at Boston University. Prior to joining Boston University, he was a CNRS researcher at the Ecole Supérieure de Physique et de Chimie Industrielles in Paris. He is a Fellow of the Optical Society (OSA) and the American Institute for Medical and Biological Engineering (AIMBE).