Immobilised Macromolecules: Application Potentials

Immobilized functional biomolecules, particularly enzymes, are important tools in biotechnology, biochemistry, biochemical engineering, biomedicine and biosensor research. This book provides an introduction and overview of selected major areas of the science and technology of immobilized systems. The chapters are intended as an introduction and overview to these interdisciplinary areas, as well as a source of practical details and of new research trends. This book will be useful for scientists, technologists, academics and students in direct and related fields.

1 Protein Stability, Folding and Association.- 2 Immobilisation of Macromolecules and Cells.- 3 Immunoaffinity Chromatography and Oriented Immobilisation of Antibodies.- 4 Avidin-Biotin Immobilisation Systems.- 5 Development of Affinity Membranes.- 6 Crystalline Protein Layers as Isoporous Molecular Sieves and Immobilisation and Affinity Matrices.- 7 Interactions of Proteins with Biomimetic Dye-Ligands: Development and Application in the Purification of Proteins.- 8 Biosensors — a Device Oriented Application of Immobilised Enzymes and Antibodies.- 9 New Immobilisation Techniques for the Preparation of Thin Film Biosensors.- 10 Two-dimensional (Glyco)protein Crystals as Patterning Elements and Immobilisation Matrices for the Development of Biosensors.- 11 Immobilised Enzymes in Optical Biosensors.- 12 Immobilisation of Macromolecules for Obtaining Biocompatible Surfaces.- 13 Crystalline Bacterial Cell Surface Layers (S-Layers) as Combined Carrier/Adjuvants for Conjugate Vaccines.