A common method of chemical analysis, modern high-performance (or high-pressure) liquid chromatography (HPLC) has many applications, most notably in the pharmaceutical industry, where it is often coupled with mass spectrometry for definitive identification of compounds. HPLC: A Practical User's Guide, Second Edition is the perfect companion for the novice chromatographer who needs to keep an HPLC system up and running, and to get the most out of the bewildering variety of column types available for making separations. This new edition brings the guide up to date with information on important advances, while maintaining the proven techniques and writing that made the first edition a useful teaching tool and reference.