Histopathology Methods and Protocols

Histopathology: Methods and Protocols provides a comprehensive guide to the current issues in histopathology. With chapters on organ-based approaches with specific protocols for morphologic, molecular examination and pathological observations governing the therapeutic management of the diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Histopathology: Methods and Protocols seeks to be a useful reference for pathologists, pathology residents and fellows as well as to the clinicians and scientists.

Gross Examination.- The Pathologist’s Guide to Fixatives.- Tissue Processing and Hematoxylin and Eosin Staining.- Light Microscope Alignment Methods.- Immunohistochemistry in Surgical Pathology: Principles and Practice.- Optimizing Direct Immunofluorescence.- Electron Microscopy: A Brief History and Review of Current Clinical Application.- A Practical Approach to Clinical and Research Bio banking.- Hospital Autopsy Performance and Reporting.- Characterization of HER2 Status by Fluorescence in situ Hybridization (FISH) and Immunohistochemistry (IHC).- Intraoperative Frozen Section Consultation in Gynecologic Pathology.- Processing Dermatological Biopsies.- Human Papillomavirus Testing in Head and Neck Squamous Cell Carcinoma: Best Practice for Diagnosis.- Peripheral Blood Smears, Bone Marrow Aspiration, Trephine and Clot Biopsies: Methods and Protocols.- Processing the Lymph Node Biopsy.- Handling and Interpretation of Heart Transplant Biopsies.- Handling And Interpretation Of Lung Transplant Biopsies.- Liver: Tissue Handling and Evaluation.- Handling and Interpretation of the Medical Renal Biopsy.- Optimizing Prostate Specimen Handling for Diagnosis and Prognosis.- Optimal Handling of Bone Tumor Specimens.- CNS Intraoperative Consultation: A Survival Guide for Non-Neuropathologists.- Examining and Processing Eye Specimens.- Optimizing the Skeletal Muscle Biopsy.