Histopathology Methods and Protocols

Histopathology: Methods and Protocols provides a comprehensive guide to the current issues in histopathology. With chapters on organ-based approaches with specific protocols for morphologic, molecular examination and pathological observations governing the therapeutic management of the diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Histopathology: Methods and Protocols seeks to be a useful reference for pathologists, pathology residents and fellows as well as to the clinicians and scientists.

Part I

1. Gross Examination

Stephen A. Geller

2. The Pathologist’s Guide to Fixatives

Kiran Qidwai , Michelle Afkhami  and Christina Day

3. Tissue Processing and Hematoxylin and Eosin Staining

Ada T. Feldman

4. Light Microscope Alignment Methods

Robert Bagnell

5. Immunohistochemistry in Surgical Pathology: Principles and Practice

Clive R Taylor

6. Optimizing Direct Immunofluorescence

Ian D. Odell and Deborah Cook

7. Electron Microscopy:  A Brief History and Review of Current Clinical Application

Ronald E. Gordon

8. A Practical Approach to Clinical and Research Biobanking

William H. Yong, Sarah M. Dry, and Maryam Shabihkhani

Part II

9. Hospital Autopsy Performance and Reporting

Jacqueline Benjamin

10. Characterization of HER2 Status by Fluorescence in situ Hybridization (FISH) and Immunohistochemistry (IHC)

Oliver A. Press, Roberta Guzman, Monica Cervantes, Angela Santiago, and Michael F. Press

11. Intraoperative Frozen Section Consultation in Gynecologic Pathology

Fabiola Medeiros and Juan C. Felix

12. Processing Dermatological Biopsies

Gene Kim^, Brittney DeClerck, and Eric Sorenson

13. Human Papillomavirus Testing in Head and Neck Squamous Cell Carcinoma: Best Practice for Diagnosis

Andrew Schache, James Croud, Max Robinson, and Selvam Thavaraj

14. Peripheral Blood Smears, Bone Marrow Aspiration, Trephine and Clot Biopsies:

Methods and Protocols

Michelle Afkhami, Maria Vergara-Lluri, Russell K. Brynes, and Imran N. Siddiqi

15. Processing the Lymph Node Biopsy

Eric Loo and Imran Siddiqi

16. Handling and Interpretation of Heart Transplant Biopsies

Eric Swanson and W. Dean Wallace

17. Handling And Interpretation Of Lung Transplant Biopsies

Eric Swanson and W. Dean Wallace

18. Liver:  Tissue Handling and Evaluation

Stephen A. Geller

19. Handling and Interpretation of the Medical Renal Biopsy

Michael N. Koss

20. Optimizing Prostate Specimen Handling for Diagnosis and Prognosis

David G. Bostwick, Christina Day, and Isabelle Meiers

21. Optimal Handling of Bone Tumor Specimens

Leslie G. Dodd, Shi Wei, and Gene P. Siegal

22. CNS Intraoperative Consultation:  A Survival Guide for Non-Neuropathologists

Jesse Lee Kresak, Marie Rivera-Zengotita, Robin M. Foss, Anthony T. Yachnis

23. Examining and Processing Eye Specimens

Amir A. Azari ,Nasreen A. Syed, and Daniel M. Albert

24. Optimizing the Skeletal Muscle Biopsy

Karen M. Weidenheim