HDAC/HAT Function Assessment and Inhibitor Development Methods and Protocols

Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models.- Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells.- Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes.- Development of a Cellular Model Mimicking Specific HDAC Inhibitors.- Evaluation of Anti-Tumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model.- Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation.- Investigating Physiopathological Roles for Sirtuins in a Mouse Model.- Patient-Derived Organoids for In Vivo Validation of In Vitro Data.- Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges.- Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1-3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines.- Evaluation of Small Molecule HDAC Inhibitors through In Vitro and In Cellulo Approaches.- Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras.- HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles.- Synthesis and Characterization of Reversible Covalent HDAC4 Inhibitors.- Analysis of Cell Cycle and DNA Compaction Dependent Subnuclear Distribution of Histone Marks.- Analyzing the Effects of HDAC Inhibitors on DNA Damage and Associated Cytotoxicity in Primary Hepatocytes.- Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) Stress.- Assessment of Mitochondrial Dysfunctions after Sirtuin Inhibition.- Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Non-Homologous End Joining.- Assessing SIRT7 Activity In Vivo and In Vitro in Response to DNA Damage.- Methods to Evaluate the Effects of HAT/KAT Inhibition on SIAH2-Driven Reactive Oxygen Species Generation in Helicobacter pylori-Infected Gastric Epithelial Cells.- Monitoring Changes in Intracellular Reactive Oxygen SpeciesLevels in Response to Histone Deacetylase Inhibitors.- Single-Cell Analysis of Histone Acetylation Dynamics at Replication Forks Using PLA and SIRF.- Testing the Effect of Histone Acetyltransferases on Local Chromatin Compaction.- A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virotherapy.- Cloning Strategy for HDAC1/HDAC2 Hybrid Protein Expression in Mammalian Cells.- Continuous Histone Deacylase Activity Assays.- CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor Resistant Cells.- Determining Potency of Inhibitors Targeting Histone Deacetylase 6 by Quantification of Acetylated Tubulin in Cells.- Expression and Crystallization of HDAC6 Tandem Catalytic Domains.- Fluorescence Polarization-Based Competition Assays to Evaluate Histone Deacetylase 6 Inhibitors.- Immunoprecipitation of HDAC6 and Interacting Proteins.