Handbook of Dynein

Dyneins are biological molecular motors that move along microtubules and drive a surprisingly wide range of essential cellular processes. This book offers information on both cytoplasmic and flagellar dyneins and the proteins they directly interact with, combining data obtained with a range of biophysical, biochemical and cell biological methods.

This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give readers a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. This is a useful handbook for frontline researchers as well as a textbook for advanced students. Besides comprehensive cover of structural information gained by electron microscopy, electron cryo-tomography, X-ray crystallography, and nuclear magnetic resonance, this book provides detailed descriptions of mechanistic experiments by single-molecule nanometry. The reports include in vitro studies of the motility of reconstituted complexes and in vivo studies of organisms expressing mutant dyneins. The recent technical improvements described, which have played an important part in recent advances, include the expression and preparation of recombinant dynein heavy chains or individual subdomains.

Dyneins: Ancient Protein Complexes Gradually Reveal Their Secrets, L. A. Amosand K. HiroseTwo Decades of Cytoplasmic Dynein: From Fast to Forceful, R. J. McKenney and R. B. ValleeFunctional Analysis of the Dynein Motor Domain, T. Shima, K. Sutoh, and T. KonStructural Studies on the Dynein Heavy Chain, A. J. Roberts and S. A. BurgessStructural Analysis of Microtubule-Bound Dynein, K. HiroseThe Dynein Stalk: Atomic Structure and Roles in the Mechanism of the Dynein Motor, A. P. CarterEnzymatic and Motile Properties of Native Dynein Molecules, Y. Toyoshima and H. HiguchiMotile Properties of Cytoplasmic Dynein, S. L. Reck-Peterson, R. D. Vale, and A. GennerichMotile Properties of Inner Arm Dynein, Y. Shitaka, H. Kojima, and K. OiwaDynein Motility in Cilia and Flagella, A. Gokhale, M. Wirschell, W. Sale, and D. R. Mitchell3D Structures of Axonemes, T. IshikawaFunctional Diversity of Axonemal Dyneins, R. KamiyaAssembly and Regulation of Dynein Light Chains, A. Nyarko andE. BarbarRegulatory Subunits of Axonemal Dynein, K. InabaThe Role of Dynein in Yeast Nuclear Segregation, M. D. Stuchell-Brereton, J. K. Moore, and J. A. Cooper