Preface
Acknowledgments
Self-Assembled Monolayers
Self-Assembled Monolayers: A Versatile Tool for Biofunctionalization of Surfaces Atul N. Parikh and David L. Allara
Introduction
Self-Assembly Mechanisms
Spatial Patternability
Biologically Relevant Surface Functionalization
Summary
Gemini SAMs Kaoru Tamada and Shinobu Yokokawa
Introduction
Basic Characteristics of Gemini-Structured SAMs
Selective Adsorption of Tartatic Acid
Optical Resolution on Chiral SAMs
Summary
Physical Chemistry of Nonfouling Oligo (Ethylene Oxide)-Terminated Self-Assembled Monolayers Michael Grunze
Introduction
A Survey of Physicochemical Surface Properties Relevant for Biofouling
Surface Energy
Charge
Steric Repulsion by Polymer Brushes
Preparation of OEG-Terminated Alkanethiolate SAMs and Their Characterization
Stability of OEG and PEG Coatings
OEG SAMs on Other Substrates
Mechanisms of "Inertness" of OEG SAMs
Hydration of Oligo(Ethylene Oxide)
The Thermodynamic Perspective
Conclusions
Electrochemically Designed Self-Assembled Monolayers for the Selective Immobilization and Release of Ligands, Proteins, and Cells Omar Azzaroni and Roberto C. Salvarezza
Introduction
Electrochemistry of Thiol Self-Assembled Monolayers on Metal Surfaces
Controlling Biological Activity Using Electroactive Self-Assembled Monolayers
Controlling the Release of Bioactive Elements by Electrochemical Desorption of Self-Assembled Monolayers
Conclusions
OM-CVD on Patterned SAMs Silvia Mittler
Introduction 140
OM-CVD of Cu onto Patterned MPTS and SAMs on OTS as a Blocking Resist
OM-CVD of Pd and Pt onto Patterned OTS SAMs as a Blocking Resist
OM-CVD-Grown Au on Stamped SAMs on Au/Mica
OM-CVD-Grown Gold NPs in Rows on SNOM Nanolithographically Manipulated HDT SAMs on Au
OM-CVD-Grown Au NPs on Cu– Ion Patterned OTS SAMs on Oxidized Silicon Wafers
OM-CVD-Grown Au NPs on Ga+ Ion FIB-Patterned OTS SAMs on Oxidized Silicon Wafers
OM-CVD-Grown Au NPs on Ga+ Ion FIB-Patterned OTS SAMs on Oxidized Silicon Wafers with Additional Density Control due to the Application of a Binary Mixed Refilled SAM
OM-CVD-Grown Au NPs on Ga+ Ion FIB-Patterned OTS SAMs on SiO2/ITO/Glass and Directly on Glass
Conclusion
Brushes, Dendrimers, Networks
Modification of Glass Surfaces by Phosphorus Dendrimer Layers for Biosensors Anne-Marie Caminade and Jean-Pierre Majoral
Introduction
Modification of Inorganic Surfaces by Phosphorus Dendrimers
Chemical Sensors Based on Phosphorus Dendrimers
Biosensors Based on Phosphorus Dendrimers
Conclusion
Biofunctional Dendrons Grafted on a Surface Young-Eun Choi and Joon Won Park
Introduction
Self-Assembly of Dendrons through Covalent Bonds
Self-Assembly of a Dendron through a Noncovalent Bond
Conclusion
Surface-Attached Polymeric Hydrogel Films Ulrich Jonas, Coenraad R. van den Brom, Annette Brunsen, and Robert F. Roskamp
Introduction
Hydrogel Systems
Structure and Properties of Hydrogel Layers
Applications
Conclusion and Outlook
Evanescent Wave Biosensors with a Hydrogel Binding Matrix 361 Jakub Dostalek, Yi Wang, Chun Jen Huang, and Wolfgang Knoll
Introduction
Key Characteristics of Hydrogel Binding Matrices
Evanescent Wave Optics for Probing Hydrogel Films
Mass Transport and Affinity Binding of Analyte in a Gel
Biosensor Implementations
Conclusion and Outlook
Surface Modification of High-Strength Interpenetrating Network Hydrogels for Biomedical Device Applications David Myung, Lampros Kourtis, Jaan Noolandi, Jennifer Cochran, Christopher N. Ta, and Curtis W. Frank
Introduction
Literature Review
Bulk and Surface Properties of PEG/PAA IPN Hydrogels
Cellular Interaction with Surface-Modified PEG/PAA Hydrogels
Summary and Outlook
Ultrasensitive Biosensing with Polymer Brushes Fang Yu
Introduction
Polymer Brush Matrix Based on Dextran Chemistry
Protein Immobilization on a Dextran Matrix
Comparison between 2D and 3D Matrices
LOD Evaluation
SPFS PSA Assay
Conclusion
Peptides, Proteins
Noncovalent Immobilization of Proteins to Surfaces Pascal Jonkheijm and Jurriaan Huskens
Introduction
Site-Selective Noncovalent Immobilization Methods
Conclusion and Perspectives
Recent Progress on Site-Selective Covalent Methods for Generating Protein Biochips Qi An and Pascal Jonkheijm
Introduction
Site-Selective Surface Immobilization of Proteins
Conclusion and Perspectives
S-Layer Proteins Uwe B. Sleytr, Dietmar Pum, Eva Maria Egelseer, Nicola Ilk, and Bernhard Schuster
Introduction
Location and Ultrastructure of S-Layers
Isolation, Chemical Characterization, and Molecular Biology
Assembly of S-Layers in vivo
Self-Assembly of Isolated S-Layer Subunits (in vitro)
S-Layer Ultrafiltration Membranes
S-Layers as Matrix for the Immobilization of Functional Macromolecules and Nanoparticles
S-Layer Fusion Proteins and S-Layer Neoglycoproteins
S-Layers as a Matrix for Biomineralization
S-Layer-Stabilized Planar Lipid Membranes and Liposomes
Conclusions
Peptide Nanotube Coatings for Bioapplications Lise T. de Jonge and Molly M. Stevens
Introduction
Self-Assembled Peptide-Based Nanotubes
Peptide Nanotube Coatings
Peptide Nanotubes for Bioapplications
Summary and Outlook
Sugars
Heparan Sulfate Surfaces to Probe the Functions of the Master Regulator of the Extracellular Space Nina S. Azmi and David G. Fernig
Biological Significance of Glycosaminoglycans
Heparin and Heparan Sulfate
Oligosaccharide Preparation
Strategies for Functionalizing Surfaces with HS, Heparin, and Derived Oligosaccharides
Applications of HS- and Heparin-Functionalized Surfaces
Opportunities for Heparin- and HS-Functionalized Surfaces
Conclusion
Heparanated Surfaces Victor Nurcombe, William R. Birch, and Simon M. Cool
Proteoglycans: Core Proteins and GAG Sugars (Mulloy)
Heparan Sulfate Biochemistry
The GAG Chains on Proteoglycans are "Catalysts of Molecular Encounter"
HS Functions
Glycosaminoglycans and Surfaces
The Manipulation of Signaling by Immobilized HS
Experiments on Specific Heparanated Surfaces
Conclusions
Lipid Bilayer Membranes
Biomimetic Systems: The Tethered Bilayer Lipid Membrane Stefan M. Schiller
Introduction
Models of the Biological Membrane
Components of the tBLM and Their Properties
Examples for tBLMs, Detection Schemes, and Their Applications
Current and Future Directions
Cell-Free Synthesis of Complex Membrane Proteins Ahu Arslan Yildiz, Sandra Ritz, and Eva-Kathrin Sinner
Introduction
Methods and Experimental Approaches
Incorporation of Isolated Protein
Detection of Cyt-bo3 Expression and Insertion by SPFS
Immune-Blotting Assay
Enzymatic Functionality Assay
Conclusion and Outlook
Integrin-Functionalized Artificial Membranes as Test Platforms for Monitoring Small Integrin Ligand Binding by Surface Plasmon–Enhanced Fluorescence Spectroscopy Ute Reuning, Daniela Lössner, Birgit Wiltschi, Wolfgang Knoll, and Eva-Kathrin Sinner
Surface Plasmon Resonance and Surface Plasmon–Enhanced Fluorescence Spectroscopy as Tools for Recording Binding Events to Membrane-Embedded Receptor Proteins
Development of an Integrin/Ligand-Binding Test on Artificial Membranes Established on SPR/SPFS Biosensors
SPR/SPFS Monitoring of Binding Events of Different Ligands to Membrane-Embedded Integrins
SPR/SPFS, a Promising Scientific Method for the Characterization of Transmembrane Receptor Proteins
Supported Lip
