Golgi Methods and Protocols

Live Cell Imaging of Yeast Golgi Dynamics.- Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuole.- Immunofluorescence as a Method to Study Golgi Organization in Larval Salivary Glands of Drosophila melanogaster.- Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgi.- Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells.- Generation of GM130 Conditional Knockout Mouse.- Monitoring Effects of Membrane Traffic via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts.- Immunofluorescence Microscopy of the Mammalian Golgi Apparatus.- Studying the Organization of the Golgi by Super-Resolution Microscopy.- Super-Resolution Live Imaging of Cargo Traffic Through the Golgi Apparatus in Mammalian Cells.- Studying Golgi Structure and Function by Thin Section TEM.- Algorithm for Modern Electron Microscopic Examination of the Golgi Complex.- High-Pressure Freezing Followed by Freeze-Substitution, An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking.- Negative Staining Electron Microscopy for Morpho-Functional Characterization of Purified Golgi Membranes from Mammalian Cells.- An Electron Tomographic Analysis of Giantin Deficient Golgi Proposes a New Function of the Golgin Protein Family.- Modeling the Cryo-EM Structure of the Exocyst Complex.- Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Software.- A Primer on Deep Learning-Based Cellular Image Classification of Changes in the Spatial Distribution of the Golgi Apparatus After Experimental Manipulation.- Reconstituting Vesicle Budding from the TGN and Immunoisolation of Vesicles Enriched with a Specific Cargo Client.- Common Assays in Mammalian Golgi Studies.- In Vitro Methods to Investigate the Disassembly of the Golgi Ribbon during the G2-M Transition of the Cell Cycle.- Generation and Analysis of hTERT-RPE1 VPS54 Knock-Out and Rescued Cell Lines.- Rapid COG Depletion in Mammalian Cell by Auxin-Inducible Degradation System.- Generation of Stable Cell Lines Expressing Golgi Reassembly Stacking Proteins (GRASPs) by Viral Transduction.- Quantitative Proteomics Analysis of Purified Rat Liver Golgi.- Comparative Genomics for Evolutionary Cell Biology using AMOEBAE: Understanding the Golgi and Beyond.- Common Markers and Small Molecule Inhibitors in Golgi Studies.- Visualizing Reversible Cisternal Stacking in Budding Yeast Pichia pastoris.- Methods for Studying Membrane-Proximal GAP Activity on Prenylated Rab GTPase Substrates.- Studying the Role of Lipid Geometry in COPI Vesicle Formation.- Unbiased Quantification of Golgi Scattering and Golgi-Centrosome Association.- Detection and Analysis of Microtubule Nucleator ?-Tubulin Ring Complex.- Quantification of Golgi Entry and Exit Kinetics of Protein Cargoes.- CARTS Formation Assay.- Quantification of Protein Exit at the Trans-Golgi Network.- Endosomal Transport to Lysosomes and the Trans-Golgi Network in Neurons and Other Cells: Visualizing Maturational Flux.- Immunoprecipitation and Western Blot Analysis of AP-1 Clathrin-Coated Vesicles.- Live imaging of Golgi Outposts in Drosophila Dendritic Arbors.- Targeted Protein Unfolding at the Golgi Apparatus.- Modeling N-Glycosylation: A Systems Biology Approach for Evaluating Changes in the Steady State Organization of Golgi Resident Proteins.- Simple N-Glycan Profile Analysis Using Lectin Staining, Mass Spectrometry, and GlycoMaple.- In Vitro Methods to Study the Golgi Apparatus Role in Proteoglycan and Glycosaminoglycan Synthesis.- Analysis of Golgi Protein Acetylation using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry.- Identification and Characterization of GRASP55 O-GlcNAcylation.- Assaying Sterol-Regulated ER-to-Golgi Transport of SREBP Cleavage-Activating Protein Using Immunofluorescence Microscopy.- Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software.- Analysis of Golgi Secretory Functions in Cancer.