Genetic Engineering Principles and Methods

Volume 18 explores the latest advances in recombinant DNA molecule techniques and how they are revolutionizing basic research in biology. Chapters discuss obtaining good expression of genetically engineered pest-resistant genes introduced in crop plants, cloning DNAs containing palindromes, and identifying genes by 3' terminal exon trapping and much more.

Cloning and Characterization of DNAs with Palindromic Sequences.- DNA Isolation, Manipulation and Characterization from Old Tissues.- Growth Factors and Neural Connectivity.- Gene Identification by 3? Terminal Exon Trapping.- Engineering Transgenes for use in the Mammary Gland.- Problems that can Limit the Expression of Foreign Genes in Plants: Lessons to be Learned from B.t. Toxin Genes.- Renaturation and Reconstitution of Functional Holoenzyme from Recombinant Subunits of Casein Kinase II Expressed as Inclusion Bodies in E. coli.- Plant Acyl-ACP Thioesterases: Chain-Length Determining Enzymes in Plant Fatty ACID Biosynthesis.- Genetic Engineering of an Insect Parasite.- The Stop Signal Controls the Efficiency of Release Factor-Mediated Translational Termination.- Mechanism of Replication and Copy Number Control of Plasmids in Gram-Positive Bacteria.- Pathways of Protein Remodeling by Escherichia coli Molecular Chaperones.- Pheromones and Pheromone Receptors as Mating-Type Determinants in Basidiomycetes.- Synthesis and Applications of Phosphopeptides.

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.