Gene Cloning

The ability to successfully clone genes underlies the majority of our knowledge in molecular and cellular biology.Gene Cloning introduces the diverse array of techniques available to clone genes and how they can be used effectively both in the research laboratory, to gain knowledge about the gene, and for use in biotechnology, medicine, the pharmaceutical industry, and agriculture. It shows how cloning genes is an integral part of genomics and underlines its relevance in the post-genomic age, as a tool required to test predictions of gene regulation and function made through bioinformatics. Applications of gene cloning in medicine, both for diagnosis and treatment, and in the pharmaceutical industry and agriculture, are also covered in the book. Gene Cloning takes a fresh approach to teaching molecular and cellular biology and will be a valuable resource to both undergraduates and lecturers of biological and biomedical science courses.

1. Introduction 2. Genome Organisation 3. Key Tools for Gene Cloning 4. Gene Identification and DNA Libraries 5. Screening DNA Libraries 6. Further Routes to Gene Identification 7. Sequencing DNA 8. Bioinformatics 9. Production of Proteins from Cloned Genes 10. Gene Cloning in the Functional Analysis of Proteins 11. The Analysis of the Regulation of Gene Expression 12.The Production and Uses of TransgenicOrganisms 13. Forensic and Medical Applications

Dr. Julia Lodge is Assistant Professor (Lecturer) at the School of Biosciences, University of Birmingham, UK and teaches gene cloning, genetic engineering, genomics. Her main research interest is bacterial infectious diseases.Dr. Pete Lund is Associate Professor (Senior Lecturer) at the School of Biosciences, University of Birmingham, UK and teaches cellular and molecular biology, genetics, protein structure and function. His research focuses on molecular chaperones and stress responses.Dr. Steve Minchin is Associate Professor (Senior Lecturer) at the School of Biosciences, University of Birmingham, UK and teaches genetics, gene expression, genomics. His main area of research is the regulation of gene expression.