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1. Mechanisms of Drug-Induced Cholestasis
Eva Gijbels and Mathieu Vinken
2. LC-MS/MS Analysis of Bile Acids in In Vitro Samples
Debbie Dewaele, Pieter Annaert, and Eef Hoeben
3. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis
Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, and Jan G. Hengstler
4. Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function
Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, and Pieter Annaert
5. The C-DILI™ Assay: An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity
Jonathan P. Jackson and Kenneth R. Brouwer
6. In Vivo Measurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids
Stef De Lombaerde, Sara Neyt, Christian Vanhove, and Filip De Vos
7. Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy
Tânia Sousa, Rui E. Castro, Ana Coutinho, Cecília M.P. Rodrigues, Manuel Prieto, and Fábio Fernandes
8. The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis
Reza Heidari and Hossein Niknahad
9. Measuring Apoptosis and Necrosis in Cholestatic Liver Injury
Benjamin L. Woolbright and Hartmut Jaeschke
10. Necrosome Formation and Necroptosis in Experimental Cholestasis
Marta Bento Afonso and Cecília Maria Pereira Rodrigues
11. Proteomics in Primary Biliary Cholangitis
You Li, Ruqui Tang, Patrick S.C. Leung, Eric Gershwin, and Xiong Ma
12. Transcriptomics Analysis of Cholestatic Compounds In Vitro
Céline Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, and Lysiane Richert
13. Systems Microscopy of Stress Response Pathways in Cholestasis Research
Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, and Bob van de Water
14. Mouse Models for Diseases in the Cholangiocyte Lineage
Noémi Van Hul, Urban Lendahl, and Emma R. Andersson
15. Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis
Sanne Van Campenhout, Hans Van Vlierberghe, and Lindsey Devisscher
16. 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research
Elisa Pose, Pau Sancho-Bru, and Mar Coll
17. A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis
Sujit K. Mohanty, Bryan Donnelly, Haley Temple, and Gregory M. Tiao
18. Zebrafish as a Model to Study Cholestatic Liver Diseases
Duc-Hung Pham and Chunyue Yin
19. Set-Up and Use of HepaRG Cells in Cholestasis Research
Christiane Guguen-Guillouzo and André Guillouzo
20. Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis
Sreenivasa C. Ramaiahgari and Stephen S. Ferguson
21. Establishment of Sandwich Cultures of Primary Human Hepatocytes
Eva Gijbels, Tamara Vanhaecke, and Mathieu Vinken
22. Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes
Neel Deferm, Lysiane Richert, Pieter Van Brantegem, Tom De Vocht, Bing Qi, Peter de Witte, Thomas Bouillon, and Pieter Annaert
23. Ex Vivo Model in Cholestasis Research
Ruby E.H. Karsten, Dorenda Oosterhuis, Louise A. van Wijk, and Peter Olinga
24. Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies
Carol J. Soroka, David N. Assis, and James L. Boyer
25. Use of Biliary Organoids in Cholestasis Research
Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, and Ludovic Vallier
26. In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis
Sankalp Jain and Gerhard F. Ecker
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