Epitranscriptomics Methods and Protocols

Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine.- Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons.- Visualization of Xist Long Non-Coding RNA with Fluorescent CRISPR/CAS9 System.- HAMR: High-Throughput Annotation of Modified Ribonucleotides.- High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-seq).- Direct Chemical Biotinylation of RNA 5’-Ends Using a Diazo Reagent.- Identification of Methylated Transcripts Using the TRIBE Approach.- Decoding the Atlas of RNA Modifications from Epitranscriptome Sequencing Data.- Detection of MicroRNA-Mediated Target mRNA Cleavage and 3'-Uridylation in Human Cells by a SLA-RT-PCR Analysis.- Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pipeline.- Synthesis and Evaluation of Novel Neamine-Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S RibosomalRNA.- Electron Microscope Detection of 5-Methylcytosine on DNA and RNA.- Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides.- LncVar:Deciphering Genetic Variations Associated with Long Non-Coding Genes.- Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE.- Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNA.- Detection and Quantification of Pseudouridine in RNA.- 5-Methylcytosine Analysis by RNA-BisSeq.- RNA Modification Regulatory Genes in DNA Damage.- Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA.