Epitope Mapping Protocols

Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.

B-cell Epitope Mapping.- What Is a B-Cell Epitope?.- Whole Antigen Methods.- Structural Basis of Antibody–Antigen Interactions.- Epitope Mapping of Antibody–Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy.- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen.- Epitope Mapping by Surface Plasmon Resonance.- Proteolytic Fragmentation for Epitope Mapping.- Epitope Mapping by Proteolysis of Antigen–Antibody Complexes.- Identifying Residues in Antigenic Determinants by Chemical Modification.- Epitope Mapping by Differential Chemical Modification of Antigens.- Peptide Library Approaches.- Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides.- Antibody Epitope Mapping Using SPOT™ Peptide Arrays.- Peptide Microarrays for Profiling of Modification State-Specific Antibodies.- Epitope Mapping Using Phage Display Peptide Libraries.- Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries.- Antibody Specificity Profiling on Functional Protein Microarrays.- Peptide Microarrays for Determination of Cross-Reactivity.- Profiling of Antibody Signatures in Biological Fluids..- Epitope Mapping Using Randomly Generated Peptide Libraries.- Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease.- Microarrayed Allergen Molecules for Diagnostics of Allergy.- Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays.- Antigen Expressed from Recombinant DNA.- Epitope Mapping Using Homolog-Scanning Mutagenesis.- Epitope Mapping by Region-Specified PCR-Mutagenesis.- Epitope Mapping Using Phage-Display Random Fragment Libraries.- B-Cell Epitope Prediction.- Prediction of Linear B-cell Epitopes.- T-Cell EpitopeMapping.- Molecular Recognition of Diverse Ligands by T-Cell Receptors.- Identification of Human MHC Class I Binding Peptides using the iTOPIA™? Epitope Discovery System.- T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT™? Synthesis.- High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange.- T-Cell Epitope Processing (The Epitope Flanking Regions Matter).- Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules.- T-Cell Epitope Mapping.- Identification and Validation of T-Cell Epitopes Using the IFN-? EliSpot Assay.