Epithelial Cell Culture Methods and Protocols

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This second edition volume expands on the previous edition with in-depth discussions on the rapid advancements in epithelial cell biology, and the cutting-edge research and techniques used by researchers in the field. The chapters in this book cover topics such as detailed methodologies applicable to epithelial cells derived from primates, pigs, bovines, and laboratory animals; the manipulation and differentiation of epithelial cells; and epithelial cell models in the gastroenteric system in human medicine and nutrition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Epithelial Cell Culture: Methods and Protocols, Second Edition is a valuable resource for researchers in the scientific community, educators,and students who are interested in unraveling the complexities of epithelial cell biology, cultivating curiosity, and inspiring the next generation of groundbreaking research.

Culture of Mouse Thymic Epithelial Cells in Serum-Free Medium.- Cultured Pig Thyroid Follicular Cells: Electrical Evaluation of Epithelial Integrity.- Human Minor Salivary Glands: A Readily-Available Source of Salivary Stem/Progenitor Cells for Regenerative Applications.- Salivary Organotypic Tissue Culture: An Ex-Vivo 3D Model for Studying Radiation-Induced Injury of Human Salivary Glands.- Differentiation of Pig Gastric Primary Cells into Mucus Producing Epithelial Cells.- Isolation, Culture, and Microscopic Imaging of Guinea Pig Primary Gastric Tissue Cells.- Method for Two-Dimensional Epithelial Monolayer Formation Derived from Mouse Three-Dimensional Small Intestinal Organoids.- Human Hepatic Spheroid Co-Culture Model for Assessment of Drug-Induced Liver Injury.- In Vitro Porcine (Explant) Colon Culture.- Bioencapsulation of Oocytes and Granulosa Cells.- Culturing and Differentiation of Patient-Derived Ectocervical EpithelialStem Cells using Air-Liquid Interphase and Matrigel Scaffold.- Ovine Trophoblast Cells: Cell Isolation and Culturing from the Placenta at the Early Stage of Pregnancy.- Amniotic Membrane and Amniotic Epithelial Cell Culture.- Evaluation of the Epithelial Barrier Integrity in Primary Cultures of Pig Mammary Epithelial Cells.- Bovine Skeletal Muscle Satellite Cells: Isolation, Growth, and Differentiation.- Equine Induced Pluripotent Stem Cell Culture.