Epiblast Stem Cells Methods and Protocols

In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.  These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties.

Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.

Generation and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos.- Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways.- Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by using Small Molecules.- Isolation and Culture of Rabbit Embryonic Stem Cells.- Goat Embryonic Stem-like Cell Derivation and Characterization.- Derivation and Culture of Canine Embryonic Stem Cells.- Isolation and Culture of Porcine Embryonic Stem Cells.- Isolation and Culture of Pig Epiblast Stem Cells.- Isolation and Culture of Bovine Embryonic Stem Cells.- Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos.- Chicken Embryonic Stem Cells: Establishment and Characterization.- Manipulating the Avian Epiblast and Epiblast-derived Stem Cells.- Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells through an Epiblast-like State.- Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells.- Derivation of Neural Precursors from Bovine Pre-implantation Embryos.