Epiblast Stem Cells Methods and Protocols

Establishment of Mouse Epiblast Stem Cells.- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell.- Generation of Epiblast-Like Cells.- Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence.- Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells.- Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution.- 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells.- Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells.- Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq.- A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data.- Complete Transcriptome Analysis by 5’-End Single-Cell RNA-Seq with Random Priming.- Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification.- Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-Like Cells.- Generation of Gastruloids from Epiblast-Like Cells.- Definitive Endoderm from EpiSC Aggregates in Matrigel.- In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-Like Cells (PGCLCs).- Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Human Primordial Germ Cell-Like Cells (hPGCLCs) In Vitro.- Differentiation of EpiLCs on Micro-Patterned Substrates Generated by Micro-Contact Printing.- Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence.- Modeling Epiblast Shape in Implanting Mammalian Embryos.