Eosinophils Methods and Protocols

This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. 

1. Molecular Biology of Eosinophils: Introduction

            Paige Lacy, Helene F. Rosenberg, and Garry M. Walsh

 

2. Functionally Active Eosinophil Purification from Peripheral Blood

            Kiho Son, Paige Lacy, and Manali Mukherjee

 

3. Eosinophil Purification from Peripheral Blood of Rhesus Monkeys

            Fanny Legrand

 

4. Generation of Mouse Eosinophils in Tissue Culture from Unselected Bone Marrow Progenitors

            Eric Mai, Ajinkya R. Limkar, Caroline M. Percopo, and Helene F. Rosenberg

 

5. Detection of Mouse Eosinophils in Tissue by Flow Cytometry and Isolation by Fluorescence-Activated Cell Sorting (FACS)

            Caroline M. Percopo, Ajinkya R. Limkar, Albert C. Sek, and Helene F. Rosenberg

 

6. Mutant Mice and Animal Models of Airway Allergic Disease

            Julyanne Brassard, David Marsolais, and Marie-Renee Blanchet

 

7. Eosinophils in Asthma Models to House Dust Mite for Drug Development

            François Daubeuf and Nelly Frossard

 

8. Analysis of Mouse Eosinophil Migration and Killing of Tumor Cells

            Sharon Grisaru, Michal Itan, and Ariel Munitz

 

9. Murine Models of Eosinophil Function in Fungal and Viral Infections

            Meenakshi Tiwary, Kim S. LeMessurier, and Amali E. Samarasinghe

 

10. Assays of Eosinophil Apoptosis and Phagocytic Uptake

            Naomi N. Gachanja, David A. Dorward, Adriano G. Rossi, and Christopher D. Lucas

 

11. In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation

            Emi Hamaguchi-Tsuru, Masataka Korenaga, and Fabrizio Bruschi

 

12. Observation and Quantification of Eosinophil Motility

            Karina T. Barretto, Deane F. Mosher, Nizar N. Jarjour, Colleen S. Curran, Stephane Esnault, and Mats W. Johansson

 

13. Isolation and Functional Aspects of Eosinophil-Derived Exosomes

            José Antonio Cañas, José Manuel Rodrigo-Muñoz, and Victoria del Pozo

 

14. Interactions of Eosinophils with Nerves

            Matthew G. Drake, Becky J. Proskocil, and Zhenying Nie

 

15. Measurement of Eosinophil Kinetics In Vivo

            Neda Farahi, Daniel Gillett, Chrystalla Loutsios, A. Michael Peters, Charlotte Summers, and Edwin R. Chilvers

 

16. Detection of Eosinophil Extracellular DNA Traps

            Marina Valente Barroso and Josiane S. Neves

 

17. Eosinophil Shape Change and Secretion

            Sergei I. Ochkur, Elizabeth A. Jacobsen, and Paige Lacy

 

18. Update on Eosinophils Interaction with Mast Cells: The Allergic Effector Unit

            Roopesh Singh Gangwar, Hadas Pahima, Pier Giorgio Puzzovio, and Francesca Levi-Schaffer

 

19. Assessing Phenotypic Heterogeneity in Intestinal Tissue Eosinophils

            Courtney L. Olbrich, Leigha D. Larsen, and Lisa A. Spencer

 

20. Antimicrobial Activity of Human Eosinophil Granule Proteins

            Anu Chopra and Janendra K. Batra

 

21. Experimental Modeling of Eosinophil-Associated Diseases

            Sathisha Upparahalli Venkateshaiah, Murli Manohar, Hemanth Kumar Kandikattu, and Anil Mishra