Enzymes Biochemistry, Biotechnology, Clinical Chemistry

This well-reviewed student textbook has now been updated to include techniques, such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification, and due to the rapid advances in bioinformatics/proteomics a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RP-HPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, this second edition is intended to provide an introduction to enzymology and it is a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

• Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject
• Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification
• Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy

Part 1: Structure and Function of Enzymes 1. An Introduction to Enzymes 2. The Structure of Proteins 3. The Biosynthesis and Properties of Proteins 4. Specificity of Enzyme Action 5. Monomeric and Oligomeric Enzymes

Part 2: Kinetic and Chemical Mechanisms of Enzyme-Catalysed Reactions 6. An Introduction to Bioenergetics, Catalysis and Kinetics 7. Kinetics of Single-Substrate Enzyme-Catalysed Reactions 8. Enzyme Inhibition 9. Kinetics of Multi-Substrate Enzyme-Catalysed Reactions 10. The Investigation of Active Site Structure 11. The chemical nature of enzyme catalysis 12. The Binding of Ligands to Proteins 13. Sigmoidal Kinetics and Allosteric Enzymes 14. The Significance of Sigmoidal Behaviour

Part 3: Application of Enzymology 15. Investigation of Enzymes in Biological Preparations 16. Extraction and Purification of Enzymes 17. Enzymes as Analytical Reagents 18. Instrumental Techniques Available for Use in Enzymatic Analysis 19. Applications of Enzymatic Analysis in Medicine, Forensic Science and Industry 20. Biotechnological Applications of Enzymes 21. Genomics, Proteomics and Bioinformatics