Enzyme-Mediated Ligation Methods

Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates.- Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL).- Cyclizing Disulfide-Rich Peptides using Sortase A.- Chemo-Enzymatic Synthesis of  Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1.- Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE).- Butelase 1-Mediated Ligation of Peptides and Proteins.- Trypsiligase-Catalyzed Peptide and Protein Ligation.- Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase.- Tailoring Activity and Selectivity of Microbial Transglutaminase.- SpyLigase-Catalyzed Modification of Antibodies.- Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases.- In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis.- Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation.- Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization.- BioID as a Tool for Protein Proximity Labeling in Living Cells.- N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells.- Tubulin Tyrosine Ligase-Mediated Modification of Proteins.- Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine.