Dopaminergic System Function and Dysfunction: Experimental Approaches

This volume provides a variety of technical approaches to study dopamine system function and dysfunction. Chapters guide readers through dopamine release in ex vivo and freely moving animals, multi-recording devices for in vivo simultaneous single cell and population activity,  in silico modeling of dopamine neurons activity, neuroanatomical approaches, unbiased stereology, ultrastructural analyses of dopaminergic neurons, and axonal innervation. Additionally, chapters also incorporate pharmacological tools to model neuropsychiatric diseases, novel behavioral paradigms to dissect dopamine's role in behavior, and functional imaging to follow human dopamine system development. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Comprehensive and cutting-edge, Dopamine Neurotransmission aims to be a valuable resource for researchers in various disciplines. 

Part I : Neuroanatomy

1          Ultrastructural Study of Dopaminergic Axon Terminals

            Natalie Doig and Max Larsson

2          Quantifying Dopaminergic Innervation in Rodents Using Unbiased Stereology

3          Dendrites Crossing Borders: Quantifying The Distribution of Individual Dopaminergic Neurons’ Dendritic Tree in Pars Compacta and Pars Reticulata

            Rafael Ignacio Gatica, Guillermo Lazcano, and Pablo Henny

Part II: Cell physiology

4          Characterizing Dopamine Transporter Mediated Uptake and Efflux in Brain Slices: A New Approach

            Nora Awadallah, Jose A. Pino, Dimitri N. Koutzoumis, and Gonzalo E. Torres

5          Modeling Pacemaking, Bursting and Depolarization Block in Midbrain Dopamine Neurons

            Christopher Knowlton and Carmen C. Canavier

            Marcelo Aguilar-Rivera, Rafael Gatica, Victor Azocar, Darinka Buc, and Jose Antonio Fuentealba

Part III: Models for addiction and neuropsychiatric diseases

            Cecilia Scorza, José Pedro Prieto, Sara Fabius, and Martín Galvalisi

8          Ethanol Intake On Preclinical Models: Methods of Assessment and Effects On Dopamine Signaling

            Ricardo M Pautassi, Aranza Wille-Bille, Macarena S Fernández, and María Carolina Fabio

9          Preclinical models of THC and Nicotine Exposure During Adolescent Brain Development: Modelling Neuropsychiatric Phenotypes Related to Dopaminergic Transmission

            Enzo Perez-Valenzuela and Steven R. Laviolette

10        The MAM Model to Study the Role of Dopamine in Schizophrenia

            Xihu Zhu, Daniela L. Uliana, and Anthony A. Grace

Part IV: Circuit level and behavior

11        Fast Scan Cyclic Voltammetry to Assess Dopamine Function: From Circuits to Behavior

            Philip J. Clark and Rodrigo A. España

12        Multi-circuit Recording in Animal Models of Parkinson

            Ximena Paz García-Nuñez, Carolina Astudillo-Valenzuela, and Romulo Fuentes-Flores

13        Neuroimaging Human Dopamine-Related Neurophysiology across Development

            Ashley Parr, Bart Larsen, Finn Calabro, Brenden Tervo-Clemmens, and Beatriz Luna

            Fabien Naneix and Etienne Coutureau