Part 1: DNA Vaccine Design DNA Vaccine Design Janet Brandsma Design of Plasmid DNA Constructs for Vaccines Donna L. Montgomery and Kristala Jones-Prather Vaccination with Messenger RNA Steve Pascolo A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines Petra Riedl, Nicolas Fissolo, Joerg Reimann, and Reinhold Schirmbeck In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors Weiwen Jiang, Charles Rich, and David Pisetsky Part 2: DNA Vaccine Delivery Systems Delivery of DNA Vaccines Using Electroporation Shawn Babiuk, Sylvia van Drunen Little-van den Hurk, and Lorne Babiuk Needle-Free Injection of DNA Vaccines Kanakatte Raviprakash and Kevin Porter Needle-Free Delivery of Veterinary DNA Vaccines Sylvia van Drunen Little-van den Hurk, Shawn Babiuk, and Lorne Babiuk Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery Mark Keegan and Mark Saltzman A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid Approach Dan Luo, Yougen Li, Soong Ho Um, and Yen Cu Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence Anne Doody and David Putnam Part 3: DNA Vaccine Adjuvants and Activity Enhancement Adjuvant Properties of CpG Oligonucleotides in Primates Daniela Verthelyi Complexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating Agents Petra Riedl, Joerg Reimann, and Reinhold Schirmbeck Prime Boost Strategies in DNA Vaccines C. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, and Stephen J. Kent Modifying Professional Antigen Presenting Cells to Enhance DNA Vaccine Potency C.-F. Hung, M. Yang, and T.C. Wu Replicase-Based DNA Vaccines for Allergy Treatment Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang Leitner, and Josef Thalhamer Part 4: DNA Vaccine Applications Immunological Responses of Neonates and Infants to DNA Vaccines Martha Sedegah and Stephen L. Hoffman DNA Vaccines for Allergy Treatment Richard Weiss, Sandra Scheiblhofer, and Josef Thalhamer Protection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR) Thorsten Buch and Ari Waisman Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms Akiko Iwasaki Part 5: DNA Vaccine Production, Purification, and Quality A Simple Method for the Production of Plasmid DNA in Bioreactors K. Listner, L. Kiser Bentley, and Michael Chartrain Practical Methods for Supercoiled pDNA Production John Ballantyne Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines Joachim Schorr, Peter Moritz, Astrid Breul, and Martin Scheef Large-Scale Non-Chromatographic Purification of Plasmid DNA Jason Murphy and Sangeetha Sagar Assuring the Quality, Safety and Efficacy of DNA Vaccines James S. Robertson