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saltzman mark w. (curatore); shen hong (curatore); brandsma janet l. (curatore) - dna vaccines

DNA Vaccines

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Lingua: Inglese

Humana Press

Pubblicazione: 11/2010
Edizione: Softcover reprint of hardcover 1st ed. 2006


In the early 1990s, almost 200 yr after Edward Jenner demonstrated the effectiveness of the smallpox vaccine, a new paradigm for vaccination emerged. The conventional method of vaccination required delivery of whole pathogens or structural subunits, but in this new approach, DNA or genetic information was administered to elicit an immunological response. Once it was observed that plasmid DNA delivered in vivo led to production of an encoded transgene (1), two ground-breaking studies demonstrated that immunological responses could be generated against antigenic transgenes via plasmid DNA delivered by DNA vaccination (as this approach is called) (2,3). The appe- ance of this new vaccination strategy coincided with advances in molecular biology, which provided new tools to study and manipulate the basic elements of an organism’s genome and also could also be applied to the design and production of DNA vaccines. DNA Vaccines is a major updated and enhancement of the first edition. It reviews state-of-the-art methods in DNA vaccine technology, with chapters describing DNA vaccine design, delivery systems, adjuvants, current appli- tions, methods of production, and quality control. Consistent with the approach of the Methods in Molecular Medicine series, these chapters contain detailed practical procedures on the latest DNA vaccine technology. The enthusiasm for DNA vaccine technology is made clear by the number of research studies published on this topic since the mid-1990s.


Part 1: DNA Vaccine Design DNA Vaccine Design Janet Brandsma Design of Plasmid DNA Constructs for Vaccines Donna L. Montgomery and Kristala Jones-Prather Vaccination with Messenger RNA Steve Pascolo A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines Petra Riedl, Nicolas Fissolo, Joerg Reimann, and Reinhold Schirmbeck In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors Weiwen Jiang, Charles Rich, and David Pisetsky Part 2: DNA Vaccine Delivery Systems Delivery of DNA Vaccines Using Electroporation Shawn Babiuk, Sylvia van Drunen Little-van den Hurk, and Lorne Babiuk Needle-Free Injection of DNA Vaccines Kanakatte Raviprakash and Kevin Porter Needle-Free Delivery of Veterinary DNA Vaccines Sylvia van Drunen Little-van den Hurk, Shawn Babiuk, and Lorne Babiuk Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery Mark Keegan and Mark Saltzman A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Non-Viral Hybrid Approach Dan Luo, Yougen Li, Soong Ho Um, and Yen Cu Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence Anne Doody and David Putnam Part 3: DNA Vaccine Adjuvants and Activity Enhancement Adjuvant Properties of CpG Oligonucleotides in Primates Daniela Verthelyi Complexes of DNA Vaccines with Cationic, Antigenic Peptides are Potent, Polyvalent CD8+ T Cell-Stimulating Agents Petra Riedl, Joerg Reimann, and Reinhold Schirmbeck Prime Boost Strategies in DNA Vaccines C. Jane Dale, Scott Thomson, Robert De Rose, Charani Ranasinghe, C. Jill Medveczky, Joko Pamungkas, David B. Boyle, Ian A. Ramshaw, and Stephen J. Kent Modifying Professional Antigen Presenting Cells to Enhance DNA Vaccine Potency C.-F. Hung, M. Yang, and T.C. Wu Replicase-Based DNA Vaccines for Allergy Treatment Sandra Scheiblhofer, Richard Weiss, Maximilian Gabler, Wolfgang Leitner, and Josef Thalhamer Part 4: DNA Vaccine Applications Immunological Responses of Neonates and Infants to DNA Vaccines Martha Sedegah and Stephen L. Hoffman DNA Vaccines for Allergy Treatment Richard Weiss, Sandra Scheiblhofer, and Josef Thalhamer Protection From Autoimmunity by DNA Vaccination Against T Cell Receptor (TCR) Thorsten Buch and Ari Waisman Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms Akiko Iwasaki Part 5: DNA Vaccine Production, Purification, and Quality A Simple Method for the Production of Plasmid DNA in Bioreactors K. Listner, L. Kiser Bentley, and Michael Chartrain Practical Methods for Supercoiled pDNA Production John Ballantyne Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines Joachim Schorr, Peter Moritz, Astrid Breul, and Martin Scheef Large-Scale Non-Chromatographic Purification of Plasmid DNA Jason Murphy and Sangeetha Sagar Assuring the Quality, Safety and Efficacy of DNA Vaccines James S. Robertson

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Medicine
Dimensioni: 229 x 152 mm Ø 589 gr
Formato: Brossura
Illustration Notes:XVI, 384 p. 61 illus.
Pagine Arabe: 384
Pagine Romane: xvi

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