DNA Vaccines Methods and Protocols

This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application.- Vaccination with Messenger RNA – a Promising Alternative to DNA Vaccination.- Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency.- CpG Oligonucleotides as Vaccine Adjuvants.- Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and Formulation.- Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate Model.- Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design.- Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation.- Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths.- Purification of Plasmid DNA by Multimodal Chromatography.- Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment.- Tumor-Specific CD8⁺ T Cell Responses Induced by DNA Vaccination.- Therapeutic DNA Vaccine Against HPV16-Associated Cancer.- Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery Applications.- Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field.- Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update Protocols.- Ethics of DNA Vaccines Transfer for Clinical Research.- Preparation of an Academic Clinical Trial.