DNA and RNA Origami Methods and Protocols

This volume detials diverse methodological approaches on the assembly and applications of DNA origami assemblies. Chapters guide readers through different synthetic and computational methods, isolation and structural characterization of 2D and 3D DNA origami nanoarchitectures,  nanophotonics, drug delivery, biophysics, and synthetic biology.Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, DNA and RNA Origami: Methods and Protocols aims to serve as a guideline describing the current state-of-the-art assembly methodologies and applications of DNA origami nanostructures.

DNA Origami: Recent Progress and Applications.- Design, Assembly, and Function of DNA Origami Mechanisms.- Computer-aided design and Functionalization of ssRNA Origami Structures.- Reconfigurable Two-dimensional DNA Molecular Arrays.- Two-dimensional DNA origami lattices assembled on lipid bilayer membranes.- The oxDNA coarse-grained model as a tool to simulate DNA origami.- All-atom Molecular Dynamics Simulations of Membrane Spanning DNA Origami Nanopores.- Single molecule imaging of enzymatic reactions on DNA origami.- Single-molecule nanomechanical genotyping with DNA origami-based shape IDs.- Using single molecule FRET to evaluate DNA nanodevices at work.- Parallel Functionalization of DNA Origami.- Protein Coating of DNA Origami.- Cellular uptake of DNA origami.- Binding and characterization of DNA origami nanostructures on lipid membranes.- Electrical actuation of DNA based nanomechanical systems.- Enzyme cascade reactions on DNA origami scaffold.- Aptamers as Functional Modules for DNA Nanostructures.- Production and Testing of RNA Origami Anticoagulants.