Computer-Aided Tissue Engineering Methods and Protocols

This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Biomimetic Boundary-based Scaffold Design for Tissue Engineering Applications.- Triply Periodic Minimal Surfaces (TPMS) for the Generation of Porous Architectures Using Stereolithography.- 3D Printing Of Functionally-graded Films by Controlling Process Parameters.- Photocurable Biopolymers for Coaxial Bioprinting.- Synthesis of an UV-curable Divinyl-fumarate Poly-e-Caprolactone for Stereolithography Applications.- Nanocomposite Clay-based Bioinks for Skeletal Tissue Engineering.- Additive Manufacturing using Melt Extruded Thermoplastics for Tissue Engineering.- Computer-aided Wet-spinning.- Production of Scaffolds Using Melt Electrospinning Writing and Cell Seeding.- Low Voltage Continuous Electrospinning: A Versatile Protocol for Patterning Nano-and Micro-Scared Fibers for Cell Interface.- Direct Write Deposition of Thermogels.- 3D Bioprinting of Complex, Cell-laden Alginate Constructs.- Surface Tension-assisted Additive Manufacturing of Tubular, Multicomponent Biomaterials.- Bioprinting of Complex Vascularized Tissues.- A Scaffold-free 3D Bioprinted Cartilage Model for in vitro Toxicology.