Comparative Genomics Methods and Protocols

This second edition provides new and updated chapters covering computational and mathematical techniques and concepts related to the field of comparative genomics. The topics covered in the chapters range from those that address general techniques and concepts that apply to all organisms to others that are specialized and apply to specific biological systems such as viruses, bacteria, nematodes, and insects. Well-known comparative genomics web-based platforms are also covered in specific chapters. Written in the highly successful Methods in Molecular Biology series format, many chapters include introductions to their respective topics and step-by-step comparison procedures, demonstrated on actual sets of genome sequences.

Authoritative and cutting-edge, Comparative Genomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

The theory of gene family histories.- Protein-coding gene families in prokaryote genome comparisons.- Family-free genome comparison.- Methods for pangenomic core detection.- Step-by-step bacterial genome comparison.- How to obtain and compare Metagenome-Assembled Genomes.- Comparative genome annotation.- Annotation and comparative genomics of Prokaryotic Transposable Elements.- Genome Rearrangement Analysis: Cut and Join Genome Rearrangements and Gene Cluster Preserving Approaches.- AGO, a framework for the reconstruction of ancestral syntenies and gene orders.- A guide to phylogenomic inference.- Comparative RNA Genomics.- Bioinformatic approaches for comparative analysis of viruses.- Comparative Analyses of Bacteriophage Genomes.- Comparative genomics of sex, chromosomes, and sex chromosomes in Caenorhabditis elegans and other nematodes.- Comparative evolutionary genomics in insects.- Comparative Methods for Demystifying Spatial Transcriptomics.- Comparative genomic analysis of bacterial data in BV-BRC: an example exploring antimicrobial resistance.- VEuPathDB resources: a platform for free online data exploration, integration, and analysis.- A practical approach to using the Genomic Standards Consortium MIxS reporting standard for comparative genomics and metagenomics.