Coenzyme B12 Enzymes Part A

B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in Methods in Enzymology serials
• Includes the latest information on B12 Enzymes

B₁₂ Biosynthesis and B₁₂ Chaperones

1. Biosynthesis of corrinoids

2. Guardian of cobamide diversity: probing the role of CobT in lower ligand activation in the biosynthesis of vitamin B₁₂ and other cobamide cofactors

3. Purification and Detection of Vitamin B₁₂ Analogs

4. A method for the efficient adenosylation of corrinoids

5. A method for the production, purification and liposome reconstitution of cobamide synthase

6. A method for the Isolation of ?-ribazole from Vitamin B12, and its Enzymatic Conversion to ?-ribazole 5'-phosphate

7. The human B₁₂ trafficking chaperones: CblC, CblD and ATR

8. Synthesis and applications of "antivitamin" B₁₂ derivatives

B₁₂-dependent enzyme reactions:

9. Coenzyme B₁₂-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase

10. Reactivating chaperones for coenzyme B₁₂-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase

11. Glutamate mutase and 2-methyleneglutarate mutase

12. Human B₁₂ dependent enzymes: Methionine synthase and methylmalonyl-CoA mutase

13. Heterologous production and biophysical characterisation of catabolic Nitratireductor pacificus pht-3B reductive dehalogenase

14. An unusual light-sensing function for coenzyme B₁₂ in bacterial transcription regulator CarH

I am currently Professor of Chemistry and Biological Chemistry at the University of Michigan in Ann Arbor. My research interests center on enzyme mechanisms and protein structure and design. We are currently working on a variety of research projects. In particular, we have a long-standing interest in enzymes that use free radicals to catalyze a variety of unusual chemical transformations. More recently, we have become interested in enzymes involved in hydrocarbon biosynthesis, many of which have novel mechanisms and are of practical interest for the biosynthesis of next-generation biofuels. We are also interested in understanding in molecular detail how enzymes interact with abiological surfaces as this is key to many industrial and biomedical applications where enzymes are immobilized on solid supports. More information about my research can be found here:http://www.lsa.umich.edu/chem/people/faculty/ci.marsheneilg_ci.detail