Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.

Foreword David J. Suggett, Ond?ej Prášil & Michael Borowitzka Chlorophyll Fluorescence Terminology: An Introduction Jeff Cosgrove & Michael A. Borowitzka In situ measurement of variable fluorescence transients Samuel R. Laney Overview of fluorescence protocols: Theory, basic concepts, and practice. Yannick Huot & Marcel Babin Fluorescence as a tool to understand changes in photosynthetic electron flow regulation Peter J Ralph, Christian Wilhelm, Johann Lavaud Torsten Jakob Katherina Petrou & Sven A. Kranz Microscopic measurements of chlorophyll a fluorescence kinetics. Ond?ej Komárek, Kristína Felcmanová, Eva Šetlíková, Eva Kotabová, Martin Trtílek, Ond?ej Prášil Estimating aquatic productivity from active fluorescence measurements David J. Suggett, C. Mark Moore & Richard J. Geider Taxonomic Discrimination of Phytoplankton by Spectral Fluorescence Hugh L. MacIntyre, Evelyn Lawrenz & Tammi L. Richardson Flow cytometry in phytoplankton research Heidi M. Sosik, Robert J. Olson & E. Virginia Armbrust The use of the fluorescence signal in studies of seagrasses and macroalgae Susana Enríquez & Michael A. Borowitzka Chlorophyll Fluorescence in Reef Building Corals Mark E. Warner, Michael Lesser & Peter Ralph Assessing nutrient status of microalgae using chlorophyll a fluorescence Kirsten Shelly, Daryl Holland & John Beardall The application of variable chlorophyll fluorescence to microphytobenthic biofilms Perkins, R.G, Kromkamp. J.C, Serôdio, J., Lavaud, J., Jesus, B., Mouget, J-L., Lefebvre, S. & Forster, R.M Chlorophyll Fluorescence Applications in Microalgal Mass Cultures Ji?í Masojídek, Avigad Vonshak & Giuseppe Torzillo Delayed Fluorescence Maja Berden-Zrimec, Luka Drinovec & Alexis Zrimec The study of phytoplankton photosynthesis by photoacoustics Yulia Pinchasov Grinblat &. ZvyDubinsky