Chemokine-Glycosaminoglycan Interactions Methods and Protocols

This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy.- Remote Ischemic Pre-Conditioning (RIPC).- Mouse Corneal Transplantation.- Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant.- Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade.- Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion.- In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor.- The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins.- Flow Cytometry Analysis of Immune Cell Responses.- Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence.- Protein Microarrays and Their Fabrication.- NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions.- Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions.- Isolation and Compositional Analysis of Glycosaminoglycans.- How to Design Peptides.- Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins.- Producing Biologics with Defined N-Glycosylation in Plants.- Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery.