Cerebral Cortex Development Methods and Protocols

Overview: Research on the Genetic Architecture of the Developing Cerebral Cortex in Norms and Diseases.- In Situ Hybridization of Brain Slices.- Immunohistochemistry of Brain Tissue.- Two-Photon FRET/FLIM Imaging of Cerebral Neurons.- Pre- and Post-Embedding Immunoelectron Microscopy to Analyze the Central Nervous System.- Mouse Brain Tissue Preparation for Scanning Electron Microscopy.- Simple Method for the Preparation of the Postsynaptic Density Fraction from Mouse Brain.- Reconstitution of Neuronal Motor Traffic on Septin-Associated Microtubules.- BioID Analysis of Actin-Binding Proteins.- Proteomic Analyses of the Mouse Brain.- Generation of iPS Cells using Sendai Virus Vectors.- Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders.- Generating Homogeneous Brain Organoids from Human iPSCs.- Primary Culture of Dissociated Neurons from Embryonic Cerebral Cortex.- Immunocytochemistry of Primary Cultured Cerebral Cortical Neurons.- Brain Sample Preparation after Performing In Utero Electroporation to Proliferating and Differentiated Cells in the Developing Mouse Neocortex.- Live Imaging of Migrating Neurons and Glial Progenitors Visualized by In Utero Electroporation.- Ca2+ Imaging in Immature Cortical Neurons.- In Vitro Patch-Clamp.- In Vivo Whole-Cell Recording from the Mouse Brain.- In Vivo Brain Microdialysis.- CT-Scan Data Analysis in Malformations of Cortical Development.- MRI Data Analysis in Malformations of Cortical Development.- Genomic Copy Number Analysis using Droplet Digital PCR: A Simple Method with EvaGreen Single-Color Fluorescent Design.- Locomotor Assays in Drosophila Larvae and Adult Flies.- Analyses of Genetic Regulation of the Nervous System in the Nematode Caenorhabditis elegans.- Analyses of Neural Circuits Governing Behavioral Plasticity in the Nematode Caenorhabditis elegans.- Social Behavior in Animal Models of Autism Spectrum Disorder.- Preparation of Healthy Single Neuron or Astrocyte Suspension from Adult Mouse Brain for RNA-seq.