Cell-Free Gene Expression Methods and Protocols

Best Practices for DNA Template Preparation towards Improved Reproducibility in Cell-Free Protein Production.- Simple Extract Preparation Methods for E. coli-Based Cell-Free Expression.- Preparation and Screening of Cell-Free Extract from Non-Growing Escherichia coli A19 Cells.- Cell-Free Protein Synthesis Using Pichia pastoris.- A Streptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression.- In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins.- High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems.- Cell-Free Gene Expression from DNA Brushes.- Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications.- Measurement of Transcription, Translation, and Other Enzymatic Processes during Cell-Free Expression Using PERSIA.- Cell-Free Noncanonical Redox Cofactor Systems.- Cell-Free Protein Synthesis forHigh-Throughput Biosynthetic Pathway Prototyping.- Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry.- Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus.- Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression.- Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles.- Cell-Free Synthesis Strategies to Probe Co-Translational Folding of Proteins within Lipid Membranes.- Assembly of RNA Nanostructures from Double Crossover Tiles.- Cell-Free Biosensors and AI Integration.- ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors.- Optical Sensing in Cell-Free Expression.- Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers.- Detection of Norovirus Using Paper-Based Cell-Free Systems.- A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes.- Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology.