Cell'Cell Interactions Methods and Protocols

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements.- Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences.- The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells.- Cell-Cell Interactions on Solid Matrices.- Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein ?-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function.- Nucleotide Metabolism and Cell-Cell Interactions.- Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization.- Collagen Gel Contraction Assay.- Methods to Assess Tissue Permeability.- Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy.- A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum.- Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems.- Expression Cloning of Signaling Proteins Regulated by Cell Adhesion.- Assays for the Calcium Sensitivity of Desmosomes.- Tight Junctions and Cell–Cell Interactions.- Simultaneous Immunofluorescence Detection of Cadherin and ?-Catenin in Mouse Tissue Sections.- In Vitro Neutrophil Transepithelial Migration.- The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions.- Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ?-3-Derived Lipid Mediators.- Isolation and Analysis of Lipid Rafts in Cell–Cell Interactions.