Carbon Nanotubes Methods and Protocols

Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: Methods and Protocols provides reliable, consistent protocols on the application of CNTs in molecular biology-related fields. These are of vital importance, as the commercially available CNTs differ in purity, agglomeration state, as well as length and diameter distribution, all of which have a profound influence on the dispersability and surface properties of the tubes. The volume contains detailed sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, provided by expert researchers from various fields. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Carbon Nanotubes: Methods and Protocols serves to contribute to the achievement of common standards and helps researchers to avoid discrepancies in future biology-related CNT studies.

Functionalization.- Non-covalent Attachment of Proteins to Single-Walled Carbon Nanotubes.- Covalent Conjugation of Multi-walled Carbon Nanotubes with Proteins.- Covalently Linked Deoxyribonucleic Acid with Multi-walled Carbon Nanotubes: Synthesis and Characterization.- Temperature and pH-Responsive “Smart” Carbon Nanotube Dispersions.- Toxicity.- Effects of Carbon Nanotubes on the Proliferation and Differentiation of Primary Osteoblasts.- Carbon Nanotube Uptake and Toxicity in the Brain.- In Vitro and In Vivo Biocompatibility Testing of Functionalized Carbon Nanotubes.- Real-Time Monitoring of Cellular Responses to Carbon Nanotubes.- Reducing Nanotube Cytotoxicity Using a Nano-Combinatorial Library Approach.- DNA Damage by Carbon Nanotubes Using the Single Cell Gel Electrophoresis Technique.- Trafficking.- Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry.- Cell Trafficking of Carbon Nanotubes Based on Fluorescence Detection.- Carbon Nanotubes as Intracellular Carriers for Multidrug Resistant Cells Studied by Capillary Electrophoresis–Laser–Induced Fluorescence.- Scaffolds.- Carbon Nanotube-Based Neurochips.- Effect of Carbon Nanotubes on HepG2 Adhesion and Spreading.- Biosensors.- Enzymatic Detection Based on Carbon Nanotubes.- Carbon Nanotube Biosensors Based on Electrochemical Detection.- Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors.- Detection of Biomarkers with Carbon Nanotube-Based Immunosensors.- Carbon Nanotube Biosensors with Aptamers as Molecular Recognition Elements.