Candida Species Methods and Protocols

CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans.- Plasmid-Based CRISPR–Cas9 Editing in Multiple Candida Species.- A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans.- How to Use the Candida Genome Database.- Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata.- Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans.- Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance.- Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS).- High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis.- Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library.- Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans.- In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin.- Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors.- Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy.- A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells.- A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions.- Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract.- Detection and Identification of Candida auris from Clinical Skin Swabs.- Production and Isolation of the Candida Species Biofilm Extracellular Matrix.- Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model.- Analysis of Gut Microbiome Using Fecal Samples.- Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry.- Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.- Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans.- Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis.