Cancer Vaccines Methods and Protocols

This volume provides readers with an overview on the latest advances in cancer vaccines, and the major issues that need to be addressed in their development. The chapters in this book cover recent progress in immunotherapy; methods for generating relevant mouse models and engineering viral-based vaccines; oncolytic virus; glycosylation-based vaccines; and clinical production of dendritic cell cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cancer Vaccines: Methods and Protocols is a valuable resource for expert and novice researchers and scientists who are looking to expand their knowledge or learn more about this important and developing field.

Current Landscape of Therapeutic Cancer Vaccines.- The Tumor Immune Environment: Advances in the Cancer Immunotherapy Era.- Making Universal Vaccines and Antibodies through Glycoengineering.- Dendritic Cell Cancer Vaccines: A Focused Review.- Mouse Model of Brain Tumor Resection.- Mouse Model of Viral Induced Brain Tumor and Generation of a Stable Tumor-Derived Cell Line.- Stereotaxic Genetic Perturbation of the Tumor Microenvironment in the Rodent Brain.- Nuclear Morphology-Based Assessment of Cell Fates Induced by a Microtubule Targeting Agent as a Single Treatment or Combined with an Oncolytic Virus.- Viral Vector-Based Cancer Vaccines.- Nanoparticles-Assisted Peptide Cancer Vaccine Delivery.- Biomimetic Glyconanoparticle Nanoghost Vaccine Based on Red Blood Cells.- Amphiphilic Glycoprobes for Cell Surface Engineering and Drug Delivery.- The Production and Characterization of Synthetic Glycoconjugate Vaccines.- Phage Display Isolation of VHH Antibodies Targeting Capsular Polysaccharides of Intratumor Bacteria.- Development of ELISA Assay for Antibody Detection in Cancer Vaccine Research.- Dendritic Cell Cancer Vaccines: Clinical Production for Cancer Immunotherapy.