Cancer Genomics and Proteomics Methods and Protocols

Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects.

Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization.

Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and analyze genes that are relevant to complex biological phenomena.

Reciprocal Subtraction Differential RNA Display (RSDD).- Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH).- The Application of Differential Display as a Gene Profiling Tool.- Serial Analysis of Gene Expression (SAGE).- Gene Expression Profile Analysis of Tumors.- cDNA Microarray and Bioinformatic Analysis of Nuclear Factor-?B Related Genes in Squamous Cell Carcinoma.- Gene Profiling Uncovers Retinoid Target Genes.- Complete Open Reading Frame (C-ORF) Technique.- Chromatin Immunoprecipitation Assays.- Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection.- Molecular Cytogenetic Applications in Analysis of the Cancer Genome.- Monitoring Methylation and Gene Expression in Cancer.- The Use of Phage Display Peptide Libraries for Basic and Translational Research.- Yeast and Mammalian Two-Hybrid Systems for Studying Protein-Protein Interactions.- Ribonomic and Short Hairpin RNA Gene Silencing Methods to Explore Functional Gene Programs Associated With Tumor Growth Arrest.- Surface-Epitope Masking (SEM).- Approaches for Monitoring Signal Transduction Changes in Normal and Cancer Cells.- PKR in Innate Immunity, Cancer, and Viral Oncolysis.- Analysis of Transformation and Tumorigenicity Using Mouse Embryonic Fibroblast Cells.- Analysis of Growth Properties and Cell Cycle Regulation Using Mouse Embryonic Fibroblast Cells.- Reverse Phase Protein Microarrays for Monitoring Biological Responses.- Protein Crystallization.