Brain Tumor Stem Cells Methods and Protocols

This updated volume explores the technically challenging study of brain tumor stem cells (BTSCs) with their unique capacity to self-renew, proliferate, and initiate tumor formation. The book collects up-to-date methodologies to isolate and propagate BTSCs and to study them using various cutting-edge techniques, in service of bringing us closer to translating the discoveries made from these cells into new therapeutic options for brain cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Brain Tumor Stem Cells: Methods and Protocols, Second Edition provides a comprehensive understanding of the skills and techniques needed to unlock data from this most informative subset of cells.

Brain Tumor Stem Cells: New Perspectives.- Isolation and Propagation of Brain Tumor Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Isolation and Culture of Human Neural Stem Cells.- Metabolic Assays for Brain Cancer Cells.- Synergy Assay for Screening Small Molecule Combinations in Brain Cancer Stem Cells.- Extracellular Flux Assays for the Measurement of Glycolysis and Mitochondrial Respiration in Brain Cancer Cells.- O6-Methylguanine–DNA Methyltransferase (MGMT) Promoter Methylation Analysis in Glioblastoma Patients.- Profiling Glioma Stem Cell Dynamics via 3D-based Cell Cycle Reporter Assays.- Proximity Ligation Assay.- Efficient Migration Assay for Brain Tumor Stem Cells.- Patient-Derived Glioblastoma Explants Empower Rapid and Personalized Drug Assessment: Harnessing the Potential of 3D Perfusion Bioreactors in Glioblastoma Drug Discovery.- Chimeric Antigen Receptor T Cell  Production for Brain Cancers.- CRISPR-Cas9-Guided Genetic Manipulation of Patient-Derived Brain Tumor Cells.- Unmasking Stemness and Immune Cell Markers in Brain Tumors with Immunohistochemistry.- In Vitro Models to Compare the Duration of Action of New Therapeutic Scaffolds and Drug Eluting Medical Devices.- Cleavage Under Targets & Release Using Nuclease (CUT&RUN) Protocols for the Study of Genetic and Epigenetic Regulation in Cancer.- A Toolkit for Single-Nucleus Characterization of Glioblastoma.- Using GlioVis to Explore, Analyze, and Visualize Brain Tumor Multi-Omics Datasets.- Modeling Glioma Stem Cell-Mediated Tumorigenesis Using Zebrafish Patient-Derived Xenograft Systems.- An In Vivo Model of Recurrent Glioblastoma.- Bioluminescent Imaging of Patient-Derived Brain Tumors in Live Mice.- Growth Dynamics of Brain Tumor through the Lens of MT-Weighted MRI.